A549 암세포 기인성 종양에 대한 18β-Glycyrrhetinic Acid의 항종양효과 (Antitumor Effect of 18β-Glycyrrhetinic Acid against Human Tumor Xenografts Caused by A549 Cancer Cell)

서지정보

· 발행기관 : 대한약학회
· 수록지 정보 : 약 학 회 지 / 55권 / 1호 / 39 ~ 44페이지
· 저자명 : 한용문, 김하얀, 김송이, 이주희

초록

Many reports indicate that 18β-glycyrrhetinic acid (18β-GA) from Glycyrrhizae Radix has anti-inflammatory and immunoregulatory activities, whereas reports regarding anticancer activity of the compound are few. In present study, we investigated antitumor effect of 18β-GA on tumor caused by A549 cancer cell in mice. Data resulting from the cytotoxicity assay showed that 18β-GA caused killing of A549 cells. LD50 values of 18β-GA were app. 180 μM and 80 μM, corresponding to 48 hr- and 72 hr-treatments, displaying that the killing activity was more effective as the 18β-GA treatment was prolonged. Based on these data, antitumor effect of 18β-GA was tested in nude mice. For induction of the tumor, A549 (3×10^6 cells/mouse) was injected subcutaneously into the lateral abdomen of nude mice (Balb/c nu/nu). To determine the antitumor effect, nude mice with tumor were given 18β-GA (1 mg/200 μl/mouse) intraperitoneally every three days for four times. Tumor-sizes were measured with a caliper for a period of 24 days. Results showed that the 18β-GA treatment reduced the tumor-sizes (P<0.05) as compared with negative control nude mice that received diluent (DPBS). The reduction degree was greater than reduction degree by doxorubicin (60 μg/mouse), and the pattern of reduction was almost sustained during the entire period of the observation. In conclusion, our studies demonstrate that 18β-GA has antitumor activity to the A549 cancer cell-caused tumor.

영어초록

Many reports indicate that 18β-glycyrrhetinic acid (18β-GA) from Glycyrrhizae Radix has anti-inflammatory and immunoregulatory activities, whereas reports regarding anticancer activity of the compound are few. In present study, we investigated antitumor effect of 18β-GA on tumor caused by A549 cancer cell in mice. Data resulting from the cytotoxicity assay showed that 18β-GA caused killing of A549 cells. LD50 values of 18β-GA were app. 180 μM and 80 μM, corresponding to 48 hr- and 72 hr-treatments, displaying that the killing activity was more effective as the 18β-GA treatment was prolonged. Based on these data, antitumor effect of 18β-GA was tested in nude mice. For induction of the tumor, A549 (3×10^6 cells/mouse) was injected subcutaneously into the lateral abdomen of nude mice (Balb/c nu/nu). To determine the antitumor effect, nude mice with tumor were given 18β-GA (1 mg/200 μl/mouse) intraperitoneally every three days for four times. Tumor-sizes were measured with a caliper for a period of 24 days. Results showed that the 18β-GA treatment reduced the tumor-sizes (P<0.05) as compared with negative control nude mice that received diluent (DPBS). The reduction degree was greater than reduction degree by doxorubicin (60 μg/mouse), and the pattern of reduction was almost sustained during the entire period of the observation. In conclusion, our studies demonstrate that 18β-GA has antitumor activity to the A549 cancer cell-caused tumor.

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