補陽還五湯 경구투여 후 면역 활성화에 의한 암 전이 억제 효과 (Anti-metastatic Effect on Cancer cell and Immune System Activation by Orally Administered Boyanghwano-tang)

서지정보

· 발행기관 : 대한한방부인과학회
· 수록지 정보 : 대한한방부인과학회지 / 27권 / 2호 / 46 ~ 58페이지
· 저자명 : 김진환, 황덕상, 이진무, 이창훈, 이경섭, 장준복

초록

Objectives: This study was designed to investigate intestinal immune systemactivation and anti-metastatic effect on cancer cells by orally administered extractsof Boyanghwano-tang.
Methods: To observe immunomodulating effects of Boyanghwano-tang on Peyer'spatch cells, we measured cytokines GM-CSF, IL-4. In addition to observing effectsof Boyanghwano-tang on hematopoiesis, we measured proliferation of bone marrowcells mediated by Peyer's patch cells in vitro.
IgA induction activated in intestinal content and serum was measured to observethe effect of orally administered Boyanghwano-tang on mucosal immune system.
After administering ovalbumin (OVA) with Boyanghwano-tang, Proliferation ofPeyer's patch cell was measured to investigate gut immunostimulatory effect.
Anti-metastatic experiments were conducted in vivo mouse model by using colon26-M3.1 carcinoma cell.
Results: The amounts of GM-CSF and IL-4 in the culture supernatant of Peyer'spatch cells were significantly increased compared to the control group. The proliferationof bone marrow cell was significantly up-regualted with Boyanghwano-tang. Theseresults indicate that oral administration of Boyanghwano-tang enhances the secretionof hematopoietic growth factors such as GM-CSF and IL-4 from Peyer's patch cells,and these cytokines also act on modulator of bone marrow cell proliferation.
After orally administering OVA with Boyanghwano-tang, IgA induction and Proliferationof peyer's patch cell was up-regulated with Boyanghwano-tang. These results meansorally administered Boyanghwano-tang activates intestinal immune system andhas an inhibitory effect on tumor metastasis.
In addition, We found that orally administered Boyanghwano-tang significantlyinhibited tumor metastasis in vivo.
Conclusions: Orally administered Boyanghwano-tang appears to have considerableactivity on the anti-metastasis by activation of immune system.

영어초록

Objectives: This study was designed to investigate intestinal immune systemactivation and anti-metastatic effect on cancer cells by orally administered extractsof Boyanghwano-tang.
Methods: To observe immunomodulating effects of Boyanghwano-tang on Peyer'spatch cells, we measured cytokines GM-CSF, IL-4. In addition to observing effectsof Boyanghwano-tang on hematopoiesis, we measured proliferation of bone marrowcells mediated by Peyer's patch cells in vitro.
IgA induction activated in intestinal content and serum was measured to observethe effect of orally administered Boyanghwano-tang on mucosal immune system.
After administering ovalbumin (OVA) with Boyanghwano-tang, Proliferation ofPeyer's patch cell was measured to investigate gut immunostimulatory effect.
Anti-metastatic experiments were conducted in vivo mouse model by using colon26-M3.1 carcinoma cell.
Results: The amounts of GM-CSF and IL-4 in the culture supernatant of Peyer'spatch cells were significantly increased compared to the control group. The proliferationof bone marrow cell was significantly up-regualted with Boyanghwano-tang. Theseresults indicate that oral administration of Boyanghwano-tang enhances the secretionof hematopoietic growth factors such as GM-CSF and IL-4 from Peyer's patch cells,and these cytokines also act on modulator of bone marrow cell proliferation.
After orally administering OVA with Boyanghwano-tang, IgA induction and Proliferationof peyer's patch cell was up-regulated with Boyanghwano-tang. These results meansorally administered Boyanghwano-tang activates intestinal immune system andhas an inhibitory effect on tumor metastasis.
In addition, We found that orally administered Boyanghwano-tang significantlyinhibited tumor metastasis in vivo.
Conclusions: Orally administered Boyanghwano-tang appears to have considerableactivity on the anti-metastasis by activation of immune system.

참고자료

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