구강암 세포주의 이소위 누드마우스 종양 모델 (AN ATOPIC NUDE MOUSE MODEL OF ORAL CANCER CELL LINE)

서지정보

· 발행기관 : 대한구강악안면외과학회
· 수록지 정보 : 대한구강악안면외과학회지 / 35권 / 2호 / 74 ~ 82페이지
· 저자명 : 김종현, 황영선, 김현실, 남웅, 차인호

초록

In order to make successful oral cancer treatment, we need to understand about tumor biology and effective chemotherapeutic agents. To achieve
these studies, it is necessary to develope a proper in-vivo model. Therefore the author will make try to develop more improved animal model of more
applicable in various method of cancer study.
In this study, the author induced in-vivo tumorigenesis in nude mice by YD-10Bmod cell line used by YD-10B cell line originated from oral tongue
squamous cell carcinoma and observed tumor formations and invasiveness of surrounding tissue, and found some results as follows :
1. The experimental group (YD-10Bmod, subcutaneous injection) produced tumors 13 out of 15 mice, while the control group produced none of 5
mice.
2. The inoculation of 1×106cells/mouse produced tumors 3 out of 5 mice and inoculation of 1×107cells/mouse, 2×107cells/mouse produced
tumors in every 5 mice.
3. In the histopathologic studies, the inoculation of 1×106cells/mouse group showed the characteristic features of well-differentiated squamous cell
carcinoma and demarcated expansile growth, while the inoculation of 1×107cells/mouse, 2×107cells/mouse group showed the expansile growth
with partial central necrosis and invasive growth to surrounding fat & connective tissue.
These findings suggest that atopic xenograft of YD-10Bmod cell line in nude mice has a improved productivity of tumors, produced tumors showed
the characteristics feature of human tumor and invasive growth to surrounding tissue in histopathologic appearance. These atopic nude mouse model
of tongue carcinoma might assist in studying oral cancer biology and effective choice of chemotherapeutic agents.

영어초록

In order to make successful oral cancer treatment, we need to understand about tumor biology and effective chemotherapeutic agents. To achieve
these studies, it is necessary to develope a proper in-vivo model. Therefore the author will make try to develop more improved animal model of more
applicable in various method of cancer study.
In this study, the author induced in-vivo tumorigenesis in nude mice by YD-10Bmod cell line used by YD-10B cell line originated from oral tongue
squamous cell carcinoma and observed tumor formations and invasiveness of surrounding tissue, and found some results as follows :
1. The experimental group (YD-10Bmod, subcutaneous injection) produced tumors 13 out of 15 mice, while the control group produced none of 5
mice.
2. The inoculation of 1×106cells/mouse produced tumors 3 out of 5 mice and inoculation of 1×107cells/mouse, 2×107cells/mouse produced
tumors in every 5 mice.
3. In the histopathologic studies, the inoculation of 1×106cells/mouse group showed the characteristic features of well-differentiated squamous cell
carcinoma and demarcated expansile growth, while the inoculation of 1×107cells/mouse, 2×107cells/mouse group showed the expansile growth
with partial central necrosis and invasive growth to surrounding fat & connective tissue.
These findings suggest that atopic xenograft of YD-10Bmod cell line in nude mice has a improved productivity of tumors, produced tumors showed
the characteristics feature of human tumor and invasive growth to surrounding tissue in histopathologic appearance. These atopic nude mouse model
of tongue carcinoma might assist in studying oral cancer biology and effective choice of chemotherapeutic agents.

참고자료

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