대장암에서 RhoA의 발현과 임상적 의의 (Expression of RhoA in Colorectal Cancers and Its Clinicopathological Significance)

서지정보

· 발행기관 : 대한대장항문학회
· 수록지 정보 : Annals of Coloproctolgy / 24권 / 6호 / 460 ~ 466페이지
· 저자명 : 정해일, 최윤영, 백무준, 배상호, 배상병, 정동준, 김성용, 이문수, 조무식, 김창호

초록

Purpose: The Rho family of GTPases are involved in
actin cytoskeleton organization and are associated
with carcinogenesis and progression of human
cancers. The clinicopathological significance of RhoA
is not yet well known in the case of colorectal cancer.
To investigate the expression of RhoA protein in colorectal
carcinoma and to evaluate the relationship
between RhoA protein expression and invasion and
metastasis of colorectal cancer, we examined the expression
of RhoA protein by using Western blotting
and immunohistochemistry. Methods: The protein
levels of RhoA in colorectal carcinomas of surgical
specimens were analyzed in 71 consecutive patients
with colorectal cancers by using immunohistochemistry
and Western blotting. The relationships between
the protein levels of RhoA in tumor tissues and the
clinicopathological features of the patients were also
assessed. Results: RhoA was highly expressed in 48
colorectal carcinomas (67.6%). There was a significant
association between RhoA expression and
lymph nodal status. The expression of RhoA protein
was related to lymph-node metastasis (P=0.032) and
advanced TNM tumor staging (P=0.020). RhoA expression
had a significant prognostic value for overall
survival. Kaplan-Meier plots of survival in patients
with high RhoA showed that high RhoA expression
was associated with a shorter overall survival.
However, no association was found between RhoA
and other pathologic or clinical variables, including
age, gender, degree of differentiation, and presence
of perineural spread. Conclusions: The RhoA protein
may be related to malignant transformation and development
of colorectal caricinomas and may play an
important role in the invasion and the metastasis of
colorectal carcinomas.

영어초록

Purpose: The Rho family of GTPases are involved in
actin cytoskeleton organization and are associated
with carcinogenesis and progression of human
cancers. The clinicopathological significance of RhoA
is not yet well known in the case of colorectal cancer.
To investigate the expression of RhoA protein in colorectal
carcinoma and to evaluate the relationship
between RhoA protein expression and invasion and
metastasis of colorectal cancer, we examined the expression
of RhoA protein by using Western blotting
and immunohistochemistry. Methods: The protein
levels of RhoA in colorectal carcinomas of surgical
specimens were analyzed in 71 consecutive patients
with colorectal cancers by using immunohistochemistry
and Western blotting. The relationships between
the protein levels of RhoA in tumor tissues and the
clinicopathological features of the patients were also
assessed. Results: RhoA was highly expressed in 48
colorectal carcinomas (67.6%). There was a significant
association between RhoA expression and
lymph nodal status. The expression of RhoA protein
was related to lymph-node metastasis (P=0.032) and
advanced TNM tumor staging (P=0.020). RhoA expression
had a significant prognostic value for overall
survival. Kaplan-Meier plots of survival in patients
with high RhoA showed that high RhoA expression
was associated with a shorter overall survival.
However, no association was found between RhoA
and other pathologic or clinical variables, including
age, gender, degree of differentiation, and presence
of perineural spread. Conclusions: The RhoA protein
may be related to malignant transformation and development
of colorectal caricinomas and may play an
important role in the invasion and the metastasis of
colorectal carcinomas.

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