D-Pinitol의 유방암 증식 및 재발 억제 효능 (Inhibitory Effect of D-pinitol on Both Growth and Recurrence of Breast Tumor from MDA-MB-231 Cancer Cells)

서지정보

· 발행기관 : 한국생약학회
· 수록지 정보 : 생약학회지 / 45권 / 2호 / 174 ~ 180페이지
· 저자명 : 김윤섭, 박지성, 김민지, 황방연, 이종길, 송석길

초록

D-Pinitol, an anti-diabetic substance, is a naturally occurring compound found in legumes. In this study, we investigatedthe inhibitory effect of D-pinitol on growth and recurrence of breast cancer. When D-pinitol was treated on MDA-MB-231 or MCF-7 breast cancer cells, it was observed that the viability of the two cancer cell lines was reduced in MTT assay.
In order to examine the effect on the growth of breast tumor, mouse xenograft assay was carried out. On day 0, nine millionscells of MDA-MB-231 were injected subcutaneously into nude mouse and D-pinitol was administered orally at the dose of500 mg/kg or 1000 mg/kg body weight for consecutive 45 days. Tumor size was reduced in dose-dependent manner upto 95.4%in 1000 mpk-treated group, compared with the non-treated control group. When D-pinitol was co-administrated with 4 µg ofdoxorubicin, recurrence of breast tumor was delayed by two weeks, compared with the mouse group of doxorubicin monotherapy.
Consistent with this data, it was observed that the population of cancer stem cells (CSCs), responsible for recurrenceof cancer, within tumor mass was significantly reduced. Taken together, D-pinitol inhibits the growth of breast cancer andrelapse of the tumor by suppressing the proliferation of CSCs.

영어초록

D-Pinitol, an anti-diabetic substance, is a naturally occurring compound found in legumes. In this study, we investigatedthe inhibitory effect of D-pinitol on growth and recurrence of breast cancer. When D-pinitol was treated on MDA-MB-231 or MCF-7 breast cancer cells, it was observed that the viability of the two cancer cell lines was reduced in MTT assay.
In order to examine the effect on the growth of breast tumor, mouse xenograft assay was carried out. On day 0, nine millionscells of MDA-MB-231 were injected subcutaneously into nude mouse and D-pinitol was administered orally at the dose of500 mg/kg or 1000 mg/kg body weight for consecutive 45 days. Tumor size was reduced in dose-dependent manner upto 95.4%in 1000 mpk-treated group, compared with the non-treated control group. When D-pinitol was co-administrated with 4 µg ofdoxorubicin, recurrence of breast tumor was delayed by two weeks, compared with the mouse group of doxorubicin monotherapy.
Consistent with this data, it was observed that the population of cancer stem cells (CSCs), responsible for recurrenceof cancer, within tumor mass was significantly reduced. Taken together, D-pinitol inhibits the growth of breast cancer andrelapse of the tumor by suppressing the proliferation of CSCs.

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