건강한 신생아에서 장내 Lactobacillus의 변화 (The Change in Intestinal Lactobacillus Count among Healthy Neonates)

서지정보

· 발행기관 : 대한소아청소년과학회
· 수록지 정보 : Clinical and Experimental Pediatrics / 47권 / 8호 / 833 ~ 838페이지
· 저자명 : 이현주, 이현지, 조수진, 서정완, 이승주, 박은애

초록

목 적 : 장내 세균총의 변화를 유발하여 염증성, 감염성, 종양성 장질환뿐 아니라 알레르기성 질환, 간질환, 고지혈증, 비뇨생식기계의 감염 등에 긍정적인 효과를 보여 전반적인 건강증진의 측면에서 probiotics의 예방적, 치료적 사용이 주목받고 있는 시점에서 질환이 있는 신생아에서 probiotics 치료의 기초 자료로 삼고자 건강한 만삭 신생아에서 장내 lactobacillus의 변화에 대한 연구를 시행하였다.방 법 : 이화의대 목동병원에 2003년 5월부터 7월까지 3개월간 모자동실에 있던 40명의 건강한 만삭 신생아를 대상으로 하였다. 모자동실에서 퇴실할 무렵인 생후 2일째, 또는 생후 5일째 대변검체를 채취하여 LCC를 측정하였다. 대상아의 성별, 재태연령, 분만형태, 수유방법, 수유량(또는 수유횟수), 대변 양상과 횟수, 구토나 설사 등의 위장관 증상을 기록하였다. LCC는 신선한 대변 검체를 채취하여 lactobacillus 선택배지에 37℃의 온도, 혐기성 상태에서 48시간 동안 배양하여 측정하였다.

영어초록

Purpose:Enhancing overall health by preventive and therapeutic use of probiotics has shown great promise on inflammatory, infectious, neoplastic disorders, allergies, et al. To attain preliminary data for the use of probiotics, we have studied the change in intestinal flora, Lactobacillus count among healthy, full term neonates.
Methods:Fresh stool samples were collected on the 2nd or 5th day of life in forty healthy full term neonates born at Ewha Womans University Hospital. Geographic factors, feeding type and gastrointestinal manifestations such as vomiting or diarrhea were recorded. Stool samples were innoculated for 48 hours in a Lactobacillus selective media in anaerobic conditions at 37℃, and the Lactobacillus colonies were counted.
Results:The lactobacillus colony count(LCC) was significantly higher in the exclusively breast milk fed neonates than those fed with a mixture of breast milk and formula(P=0.0017). LCC was significantly higher in the stool samples collected on the 5th postnatal day than the 2nd postnatal day(P= 0.0059). There were no significant differences in the LCC by sex or delivery type, and there were no significant correlations in the LCC according to the gestational age, birth weight, frequency of breast feeding, amount of milk feeding or frequency of defecation.
Conclusion:These results suggest that breast feeding provides a more favorable environment than mixed feeding with formula for the Lactobacillus growth, and LCC increases with time after birth. Considering the fact that one of the main causes of necrotizing enterocolitis is the disruption of intestinal normal flora, the result of this study can be used as a preliminary data for investigating the effect of probiotics in neonates.

참고자료

· 없음