황련해독탕 및 발효황련해독탕의 항염증 효과 (Anti-inflammatory effects of Hwangnyeonhaedok-tang and Fermented Hwangnyeonhaedok-tang)

서지정보

· 발행기관 : 대한한방안이비인후피부과학회
· 수록지 정보 : 한방안이비인후피부과학회지 / 24권 / 2호 / 1 ~ 15페이지
· 저자명 : 양현주, 주현아, 백상철, 박진수, 홍석훈

초록

Objective : Recently interest of fermented herb medicine has increased in Oriental medicine. Herb medicine fermented in a proper condition resultingly has better or new virtue by microbe. This study was designed to compare the anti-inflammatory effects of herbal decoction and fermented herbal decoction in Hwangnyeonhaedok-tang (Huanglian fiedu -tang; HHT).
Methods : The effects of HHT and FHHT on anti-inflammation were measured by production of NO and pro-inflammatory cytokine productions including TNF-α and IL-1β, IL-6 in LPS induced RAW 264.7 cells.
Results : 1. Cell viability in three doses of HHT extract (0.01, 0.05 and 0.10 ㎎/㎖) and two doses of FHHT extract (0.01, 0.05 ㎎/㎖) was more than 70％ and 80％ each.
2. In RAW 264.7 cells, LPS displayed significant stimulatory effects on the production of NO.
However, both HHT, FHHT showed significant inhibitory effects on the production of NO.
3. Pre-treatment by HHT in a various concentrations (0.01, 0.05, 0.10, 0.50㎎/㎖) reduced the elevated production of TNF-α, IL-1β , IL-1β mRNA , IL-6 mRNA. More than 0.05㎎/㎖concentrations of HHT reduced the production of IL-6. And TNF-α,IL-1β level of mouse serum reduced in a various dose(0.1, 0.5, 1.0ｇ/㎏).
4. Pre-treatment by FHHT in a various concentrations (0.01, 0.05, 0.10, 0.50㎎/㎖) reduced the elevated production of TNF-α. More than 0.05㎎/㎖ concentrations of FHHT reduced the expression of TNF-α mRNA. 0.1, 0.50㎎/㎖ concentrations of FHHT reduced the production of IL-6. And IL-1β level of mouse serum reduced in a various dose(0.1, 0.5, 1.0ｇ/㎏).
Conclusions : These results indicated that both HHT and FHHT could have anti-inflammatory capacity by inhibiting the production of NO and cytokines in vitro. But HHT has more strong anti-inflammatory effects than that of FHHT in broad outlines.

영어초록

Objective : Recently interest of fermented herb medicine has increased in Oriental medicine. Herb medicine fermented in a proper condition resultingly has better or new virtue by microbe. This study was designed to compare the anti-inflammatory effects of herbal decoction and fermented herbal decoction in Hwangnyeonhaedok-tang (Huanglian fiedu -tang; HHT).
Methods : The effects of HHT and FHHT on anti-inflammation were measured by production of NO and pro-inflammatory cytokine productions including TNF-α and IL-1β, IL-6 in LPS induced RAW 264.7 cells.
Results : 1. Cell viability in three doses of HHT extract (0.01, 0.05 and 0.10 ㎎/㎖) and two doses of FHHT extract (0.01, 0.05 ㎎/㎖) was more than 70％ and 80％ each.
2. In RAW 264.7 cells, LPS displayed significant stimulatory effects on the production of NO.
However, both HHT, FHHT showed significant inhibitory effects on the production of NO.
3. Pre-treatment by HHT in a various concentrations (0.01, 0.05, 0.10, 0.50㎎/㎖) reduced the elevated production of TNF-α, IL-1β , IL-1β mRNA , IL-6 mRNA. More than 0.05㎎/㎖concentrations of HHT reduced the production of IL-6. And TNF-α,IL-1β level of mouse serum reduced in a various dose(0.1, 0.5, 1.0ｇ/㎏).
4. Pre-treatment by FHHT in a various concentrations (0.01, 0.05, 0.10, 0.50㎎/㎖) reduced the elevated production of TNF-α. More than 0.05㎎/㎖ concentrations of FHHT reduced the expression of TNF-α mRNA. 0.1, 0.50㎎/㎖ concentrations of FHHT reduced the production of IL-6. And IL-1β level of mouse serum reduced in a various dose(0.1, 0.5, 1.0ｇ/㎏).
Conclusions : These results indicated that both HHT and FHHT could have anti-inflammatory capacity by inhibiting the production of NO and cytokines in vitro. But HHT has more strong anti-inflammatory effects than that of FHHT in broad outlines.

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