Dehydrothermal Treatment로 제작한 흡수성 콜라겐 골유도재생술 차단막 (Absorbable Guided Bone Regeneration Membrane Fabricated from Dehydrothermal Treated Porcine Collagen)

서지정보

· 발행기관 : 대한악안면성형재건외과학회
· 수록지 정보 : Maxillofacial Plastic Reconstructive Surgery / 33권 / 2호 / 112 ~ 119페이지
· 저자명 : 방강미, 정한울, 김성포, 양은경, 김기호, 김성민, 김명진, 장정원, 이종호

초록

Purpose: Collagen membranes are used extensively as bioabsorbable barriers in guided bone regeneration. However, collagen has different effects on tissue restoration depending on the type, structure, degree of cross-linking and chemical treatment. The purpose of this study was to evaluate the inflammatory reaction, bone formation, and degradation of dehydrothermal treated porcine type I atelocollagen (CollaGuideⓇ) compared to of the non-crosslinked porcine type I, III collagen (BioGideⓇ) and the glutaldehyde cross-linked bovine type I collagen (BioMendⓇ) in surgically created bone defects in rat mandible.
Methods: Bone defect model was based upon 3 mm sized full-thickness transcortical bone defects in the mandibular ramus of Sprague-Dawley rats. The defects were covered bucolingually with CollaGuideⓇ, BioMendⓇ, or BioGideⓇ (n=12). For control, the defects were not covered by any membrane. Lymphocyte, multinucleated giant cell infiltration, bone formation over the defect area and membrane absorption were evaluated at 4 weeks postimplantation. For comparison of the membrane effect over the bone augmentation, rats received a bone graft plus different covering of membrane. A 3×4 mm sized block graft was harvested from the mandibular angle and was laid and stabilized with a microscrew on the naturally existing curvature of mandibular inferior border. After 10 weeks postimplantation, same histologic analysis were done.


원고접수일 2010년 9월 20일, 게재확정일 2011년 1월 4일책임저자 이종호(110-749) 서울시 종로구 연건동 28번지, 서울대학교 치의학대학원 구강악안면외과학교실Tel: 02-2072-2630, Fax: [phone], E-mail: [e-mail]

RECEIVED September 20, 2010, ACCEPTED January 4, 2011Correspondence to Jong-Ho Lee Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University 28, Yeongun-dong, Chongro-gu, Seoul 110-749, Korea Tel: 82-2-2072-2630, Fax: [phone], E-mail: [e-mail]

CC This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Results: In the defect model at 4 weeks post-implantation, the amount of new bone formed in defects was similar for all types of membrane. Bio-GideⓇ membranes induced significantly greater inflammatory response and membrane resorption than other two membranes; characterized by lymphocytes and multinucleated giant cells. At 10 weeks postoperatively, all membranes were completely resorbed.
Conclusion: Dehydrotheramal treated cross-linked collagen was safe and effective in guiding bone regeneration in alveolar ridge defects and bone augmentation in rats, similar to BioGideⓇ and BioMendⓇ, thus, could be clinically useful.

영어초록

Purpose: Collagen membranes are used extensively as bioabsorbable barriers in guided bone regeneration. However, collagen has different effects on tissue restoration depending on the type, structure, degree of cross-linking and chemical treatment. The purpose of this study was to evaluate the inflammatory reaction, bone formation, and degradation of dehydrothermal treated porcine type I atelocollagen (CollaGuideⓇ) compared to of the non-crosslinked porcine type I, III collagen (BioGideⓇ) and the glutaldehyde cross-linked bovine type I collagen (BioMendⓇ) in surgically created bone defects in rat mandible.
Methods: Bone defect model was based upon 3 mm sized full-thickness transcortical bone defects in the mandibular ramus of Sprague-Dawley rats. The defects were covered bucolingually with CollaGuideⓇ, BioMendⓇ, or BioGideⓇ (n=12). For control, the defects were not covered by any membrane. Lymphocyte, multinucleated giant cell infiltration, bone formation over the defect area and membrane absorption were evaluated at 4 weeks postimplantation. For comparison of the membrane effect over the bone augmentation, rats received a bone graft plus different covering of membrane. A 3×4 mm sized block graft was harvested from the mandibular angle and was laid and stabilized with a microscrew on the naturally existing curvature of mandibular inferior border. After 10 weeks postimplantation, same histologic analysis were done.


원고접수일 2010년 9월 20일, 게재확정일 2011년 1월 4일책임저자 이종호(110-749) 서울시 종로구 연건동 28번지, 서울대학교 치의학대학원 구강악안면외과학교실Tel: 02-2072-2630, Fax: [phone], E-mail: [e-mail]

RECEIVED September 20, 2010, ACCEPTED January 4, 2011Correspondence to Jong-Ho Lee Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University 28, Yeongun-dong, Chongro-gu, Seoul 110-749, Korea Tel: 82-2-2072-2630, Fax: [phone], E-mail: [e-mail]

CC This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Results: In the defect model at 4 weeks post-implantation, the amount of new bone formed in defects was similar for all types of membrane. Bio-GideⓇ membranes induced significantly greater inflammatory response and membrane resorption than other two membranes; characterized by lymphocytes and multinucleated giant cells. At 10 weeks postoperatively, all membranes were completely resorbed.
Conclusion: Dehydrotheramal treated cross-linked collagen was safe and effective in guiding bone regeneration in alveolar ridge defects and bone augmentation in rats, similar to BioGideⓇ and BioMendⓇ, thus, could be clinically useful.

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