국내 원유 세균수 검사장비 박토스캔 및 박토카운트의 보정식 재평가 (Re-evaluation on conversion system of BactoScan and BactoCount for raw milk in South Korea)

서지정보

· 발행기관 : 한국동물위생학회
· 수록지 정보 : 한국동물위생학회지 / 45권 / 1호 / 39 ~ 45페이지
· 저자명 : ESeul Kim, Jin-Hwan Kim, Yeong-Seob Byun, Dasom Park, Ha-Young Kim, Soon-Seek Yoon, Jin-San Moon

초록

The total bacteria count is significantly important factor for hygienic quality in raw milk. BactoScan FMTM and BactoCount IBCTM are the automated instruments for the determination of the total bacte-rial count in raw milk. They have been used after calibration by standard plate count (SPC) method in South Korea since 2000. It is necessary to re-evaluate for total bacterial counter according to the improvement of milk quality and the change of milk quality grade. Therefore, this study was evalu-ated the conversion mode of the two machines by SPC method. We collected 921 bulk-tank milk samples throughout the concentration range of 1,000∼1,000,000 CFU/mL from June 2020 to Octo-ber 2021. As a result, when compared by the SPC value, there was a slight difference in total bacterial count in BactoScan below 10,000 CFU/mL and above 200,000 CFU/mL and in BactoCount above 100,000 CFU/mL, respectively. Therefore, the conversion factor for BactoScan and the conversion equation for BactoCount were newly adjusted based on SPC value, and then the correlation coef-ficients (R2) was 0.85 or higher. In addition, the correlation (R2) between BactoScan and BactoCount was 0.91, which means the results were high positive correlation. These results are expected to con-tribute to improving the accuracy of the automated instruments for determining of total bacterial count in raw milk.

영어초록

The total bacteria count is significantly important factor for hygienic quality in raw milk. BactoScan FMTM and BactoCount IBCTM are the automated instruments for the determination of the total bacte-rial count in raw milk. They have been used after calibration by standard plate count (SPC) method in South Korea since 2000. It is necessary to re-evaluate for total bacterial counter according to the improvement of milk quality and the change of milk quality grade. Therefore, this study was evalu-ated the conversion mode of the two machines by SPC method. We collected 921 bulk-tank milk samples throughout the concentration range of 1,000∼1,000,000 CFU/mL from June 2020 to Octo-ber 2021. As a result, when compared by the SPC value, there was a slight difference in total bacterial count in BactoScan below 10,000 CFU/mL and above 200,000 CFU/mL and in BactoCount above 100,000 CFU/mL, respectively. Therefore, the conversion factor for BactoScan and the conversion equation for BactoCount were newly adjusted based on SPC value, and then the correlation coef-ficients (R2) was 0.85 or higher. In addition, the correlation (R2) between BactoScan and BactoCount was 0.91, which means the results were high positive correlation. These results are expected to con-tribute to improving the accuracy of the automated instruments for determining of total bacterial count in raw milk.

참고자료

· 없음