식이제한이 TALLYHO/JngJ 생쥐의 표현형에 미치는 영향 (Effects of Food Restriction on Phenotypes of TALLYHO/JngJ Mouse)

서지정보

· 발행기관 : 대한당뇨병학회
· 수록지 정보 : Diabetes and Metabolism Journal / 32권 / 4호 / 304 ~ 316페이지
· 저자명 : 정원훈, 이상달, 김희연, 구승진, 천혜경, 조성환

초록

Background: Food restriction has been reported to ameliorate diabetes and obesity. In this study, we
examined the effects of the food restriction on phenotypes of TALLYHO/JngJ (TH) mouse, a recently
developed diabetic model animal.
Methods: 3 week-old TH mice were divided into 2 groups (n = 20 each for food-restricted (THR) and free-fed
(THF)) and THR mice were fed the same amount of food as normal control mice (C57BL/6, n = 20).
Bodyweight was weekly monitored till 14 weeks of age. The half of animals were sacrificed at 8 weeks of
age, and liver, kidney, and fat weight were measured. The histopathology of liver and brown fat tissues and
mRNA expression of leptin in adipose tissue were analyzed. The oral glucose tolerance test and insulin
resistance test was done at 14 weeks of age. The plasma concentrations of glucose, free fatty acid,
triglyceride, cholesterol and leptin were analyzed.
Results: The THR mice had lower body weights than the THF mice, similar to C57BL/6 mice, with reduced
fat deposition in liver and brown fat tissue. The plasma levels of glucose, triglyceride and free fatty acid
were decreased in the THR group. The THR mice, however, carried more fat than normal mice, with
increased plasma leptin concentration and leptin mRNA expression in fats and no alteration in plasma
cholesterol levels. Furthermore, the THR mice revealed glucose intolerance with impaired after-meal insulin
secretion and slight insulin resistance
Conclusion: The food restriction apparently ameliorated the obesity and diabetic phenotypes of TH mice.
However, plasma concentration of cholesterol were not improved in THR mice with increased adiposity index
and glucose intolerance, suggesting the genetically prone tendency of obesity and diabetes development in TH
mice possibly with an impairment in cholesterol metabolism. (KOREAN DIABETES J 32:304-316, 2008)

영어초록

Background: Food restriction has been reported to ameliorate diabetes and obesity. In this study, we
examined the effects of the food restriction on phenotypes of TALLYHO/JngJ (TH) mouse, a recently
developed diabetic model animal.
Methods: 3 week-old TH mice were divided into 2 groups (n = 20 each for food-restricted (THR) and free-fed
(THF)) and THR mice were fed the same amount of food as normal control mice (C57BL/6, n = 20).
Bodyweight was weekly monitored till 14 weeks of age. The half of animals were sacrificed at 8 weeks of
age, and liver, kidney, and fat weight were measured. The histopathology of liver and brown fat tissues and
mRNA expression of leptin in adipose tissue were analyzed. The oral glucose tolerance test and insulin
resistance test was done at 14 weeks of age. The plasma concentrations of glucose, free fatty acid,
triglyceride, cholesterol and leptin were analyzed.
Results: The THR mice had lower body weights than the THF mice, similar to C57BL/6 mice, with reduced
fat deposition in liver and brown fat tissue. The plasma levels of glucose, triglyceride and free fatty acid
were decreased in the THR group. The THR mice, however, carried more fat than normal mice, with
increased plasma leptin concentration and leptin mRNA expression in fats and no alteration in plasma
cholesterol levels. Furthermore, the THR mice revealed glucose intolerance with impaired after-meal insulin
secretion and slight insulin resistance
Conclusion: The food restriction apparently ameliorated the obesity and diabetic phenotypes of TH mice.
However, plasma concentration of cholesterol were not improved in THR mice with increased adiposity index
and glucose intolerance, suggesting the genetically prone tendency of obesity and diabetes development in TH
mice possibly with an impairment in cholesterol metabolism. (KOREAN DIABETES J 32:304-316, 2008)

참고자료

· 없음