흰쥐 눈물샘에서 Carbonic Anhydrase 발현에 관한 면역조직화학적 연구 (Immunohistochemical Distribution of Carbonic Anhydrase in Rat Exorbital Lacrimal Gland)

서지정보

· 발행기관 : 대한해부학회
· 수록지 정보 : Anatomy and Cell Biology / 41권 / 1호 / 39 ~ 46페이지
· 저자명 : 안민, 이송은, 남광일, 정채용, 이승원, 안규윤, 배춘상, 김백윤, 박성식

초록

There are several carbonic anhydrase (CA) isozymes, which differ in their kinetic properties, tissue
distribution, and subcellular localization. In this study, the distribution of CA isozymes I, II, IV, and IX was
investigated in the rat exorbital lacrimal gland using Western blotting analysis and immunohistochemical staining. In
the Western blotting analysis of the rat lacrimal gland, CA II and CA IX were expressed abundantly and CA IV was
expressed weakly. Hematoxylin-eosin staining of the exorbital lacrimal gland showed a multilobular tubuloacinar
gland composed of acinar and ductal cells. Immunohistochemical reaction revealed no CAI staining in acinar cells and
positive staining in intercalated and small duct cells. CA II reactivity was detected in the supranuclear cytoplasm of
acinar cells and appeared to vary between acini. The intercalated and collecting duct cells showed weak or no immunoreactivity
for CA II. CA IV was detected in the intercalated and collecting duct cells but not at the acinar cells. CA IX
was detected in the intercalated and collecting duct cells, and in only a few acinar cells. These results demonstrate the
differential distribution of CA isoenzymes in the exorbital lacrimal gland of the rat and suggest that CA II is related
mainly to the electrolyte metabolism of tears in the acinar cells and that CAs I, IV, and IX are related to the electrolyte
metabolism of tears in the duct cells.

영어초록

There are several carbonic anhydrase (CA) isozymes, which differ in their kinetic properties, tissue
distribution, and subcellular localization. In this study, the distribution of CA isozymes I, II, IV, and IX was
investigated in the rat exorbital lacrimal gland using Western blotting analysis and immunohistochemical staining. In
the Western blotting analysis of the rat lacrimal gland, CA II and CA IX were expressed abundantly and CA IV was
expressed weakly. Hematoxylin-eosin staining of the exorbital lacrimal gland showed a multilobular tubuloacinar
gland composed of acinar and ductal cells. Immunohistochemical reaction revealed no CAI staining in acinar cells and
positive staining in intercalated and small duct cells. CA II reactivity was detected in the supranuclear cytoplasm of
acinar cells and appeared to vary between acini. The intercalated and collecting duct cells showed weak or no immunoreactivity
for CA II. CA IV was detected in the intercalated and collecting duct cells but not at the acinar cells. CA IX
was detected in the intercalated and collecting duct cells, and in only a few acinar cells. These results demonstrate the
differential distribution of CA isoenzymes in the exorbital lacrimal gland of the rat and suggest that CA II is related
mainly to the electrolyte metabolism of tears in the acinar cells and that CAs I, IV, and IX are related to the electrolyte
metabolism of tears in the duct cells.

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