염증성 사이토카인의 발현 억제를 통한 홍해삼 추출물의 항염증효과 및 아토피 개선효과 (Anti-inflammatory Effects and Improving Atopic Dermatitis of Ethanol Extracts of Red Sea Cucumber)

서지정보

· 발행기관 : 한국생물공학회
· 수록지 정보 : KSBB Journal / 32권 / 4호 / 335 ~ 341페이지
· 저자명 : 전미지, 임은경, 김근태, 김가연, 이승제, 정인철, 김상용, 김영민

초록

The red sea cucumber (RSC), Apostichopus japonicus, is a species of sea cucumber in the family Stichopodidae.
It is widely distributed in China, Japan and Korea. Tumor necrosis factor-α (TNF-α) and Interferon (IFN- γ) induce inflammatory cytokines such as Thymus and activation- regulated chemokine (TARC/CCL17), Cutaneous T-cellattracting chemokine (CTACK/CCL27), interleukin-1β (IL- 1β), IL-6 and IL-8. These cytokines are increased in atopic dermatitis condition. In this study, we investigated the antiinflammatory and skin regeneration effects by RCS. To investigate cytotoxic effects in HaCaT cells, we performed MTT assay after treatment with RSC extracted by 70% EtOH. As a result, RSC extracts did not have cytotoxic effect in HaCaT cells. Also, mRNA expression levels of CCL17, CCL27, IL- β, IL-6 and IL-8 genes induced by TNF-α/IFN-γ were decreased by RSC extracts. Moreover, we confirmed the release of CCL17 and CCL27 protein through ELISA assay.
Wound healing assay and cell cycle assay were performed to identify cell regeneration effect of RSC extracts. These results demonstrated the prevention and treatment effects of RSC on skin inflammatory diseases such as atopic dermatitis.

영어초록

The red sea cucumber (RSC), Apostichopus japonicus, is a species of sea cucumber in the family Stichopodidae.
It is widely distributed in China, Japan and Korea. Tumor necrosis factor-α (TNF-α) and Interferon (IFN- γ) induce inflammatory cytokines such as Thymus and activation- regulated chemokine (TARC/CCL17), Cutaneous T-cellattracting chemokine (CTACK/CCL27), interleukin-1β (IL- 1β), IL-6 and IL-8. These cytokines are increased in atopic dermatitis condition. In this study, we investigated the antiinflammatory and skin regeneration effects by RCS. To investigate cytotoxic effects in HaCaT cells, we performed MTT assay after treatment with RSC extracted by 70% EtOH. As a result, RSC extracts did not have cytotoxic effect in HaCaT cells. Also, mRNA expression levels of CCL17, CCL27, IL- β, IL-6 and IL-8 genes induced by TNF-α/IFN-γ were decreased by RSC extracts. Moreover, we confirmed the release of CCL17 and CCL27 protein through ELISA assay.
Wound healing assay and cell cycle assay were performed to identify cell regeneration effect of RSC extracts. These results demonstrated the prevention and treatment effects of RSC on skin inflammatory diseases such as atopic dermatitis.

참고자료

· 없음