Antibacterial activity and characterization of tissue conditioner containing silver-chitosan nanocomplex

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초록

The objective of this work was to verify the antimicrobial effect and the characteristics of the tissue conditioner containing silver-chitosan nanocomplex at in vitro level. Nanocomplex was synthesized by the chemical reduction of silver nitrate and chitosan in aqueous solution with sodium borohydride as a reducing agent. Silver-chitosan nanocomplex was incorporated to tissue conditioner as powder to powder ratio with different weight percentage (%) of 0 (control), 1.0, 3.0, 5.0 and 7.0.
The antimicrobial effect was assessed by colony forming unit in retrieved two streptococcal suspensions inoculated on specimens and cytotoxicity was measured using human gingival fibroblasts by MTS assay. Surface topography, Ag ion release and ultimate tensile strength tests were conducted for mechanical validity. The significantly reduced bacterial adhesions were observed above 5.0% loading while all of tested samples did not show cytotoxicity when compared to the control. Ag ion releases were detected with the dose-dependent of initial silver loadings with gradual decreasing over time. Modified tissue conditioner revealed similar microscopic surface textures and expressed no significant tensile strength changes (P>0.01) as compared to unmodified. Within some limitations in present study, the tissue conditioner loaded by 5.0% of silver-chitosan nanocomplex can be candidate as a novel denture biomaterial without mechanical hazards. For a clinical specification, future studies including in vivo, multi-strain or factor assays, and additional physical tests were still required.

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