No Ozone Cold Plasma를 처리한 RAW 264.7 세포에서의 RANKL 유도된 초기 뼈파괴세포 분화 증가 (RANKL-Induced Early Osteoclast Differentiation Increase in RAW 264.7 Cells Treated with No Ozone Cold Plasma)

서지정보

· 발행기관 : 대한통합의학회
· 수록지 정보 : 대한통합의학회지 / 13권 / 1호 / 29 ~ 37페이지
· 저자명 : 최별보라, 김지영, 박상례, 김규천

초록

Purpose : Orthodontics is an essential treatment method for modern people with high aesthetic concerns, as it corrects malocclusion or uneven teeth. Orthodontic treatment changes the position of the teeth by moving them. Osteoclasts are formed on the side where the correction force is applied, and osteoblasts are formed on the other side. Plasma is the fourth substance and has been widely used in various medical fields, including bacterial killing, cell differentiation, and cancer cell death. This study aimed to assess the efficiency of no ozone cold plasma (hereafter referred to as NCP), which generates little ozone and is used in various medical fields, to induce osteoclast differentiation for proper tooth movement.
Methods : This study conducted the following experiment to investigate whether the treatment of RAW 264.7 cells with no-ozone cold plasma induces differentiation into osteoclast cells. Water-soluble tetrazolium salt-1 assay was used to examine the growth of RAW 264.7 mouse macrophages, and tartrate-resistant acid phosphatase (TRAP) staining was used to evaluate NCP-induced osteoclast differentiation. Additionally, real-time polymerase chain reaction was used to measure the mRNA levels of osteoclast-related factors.
Results : In this study, RAW 264.7 cell growth was significantly lower in the NCP-treated group than in the untreated group at 24 and 48 h. TRAP staining revealed increased osteoclast differentiation of these cells. Moreover, real-time polymerase chain reaction revealed significantly higher mRNA levels of osteoclast-inducing factors c-FOS, cathepsin K, nuclear factor of activated T-cells cytoplasmic 1, osteoclast-associated receptor, and TRAP in the NCP-treated group than in the untreated group.
Conclusion : Overall, our results demonstrate that no ozone cold plasma successfully induced the osteoclast differentiation of RAW 264.7 cells, showing potential for use in novel tooth movement devices for orthodontic treatment.

영어초록

Purpose : The purpose of this study was to investigate the effect of inducing osteoclast differentiation for tooth movement by using no-ozone cold plasma which is effective and has few side effects.
Methods : To performed the wst-1 assay to confirm cell growth rate and TRAP stain to specifically stain osteoclasts. In addition, real-time PCR was performed to measure osteoclast-inducing factors at the mRNA level.
Results : After plasma treatment, the growth rate of RAW 264.7 cells was statistically significant reduced in the treated group compared to the untreated group at 24 and 48 hours. The TRAP stain results also confirmed that the degree of osteoclast differentiation increased. In addition, at the mRNA level through real-time PCR, osteoclast-inducing factors c-FOS, CTSK, NFATc1, OSCAR, and TRAP were statistically significant increased in the treated group compared to the untreated group.
Conclusion : This study confirmed that no-ozone cold plasma treatment induces osteoclast differentiation in RAW 264.7 cells. This suggest that it can be developed into a device that can be utilized for tooth movement through orthodontic treatment in the future.

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