自然銅의 투여가 인체의 뼈모세포 활성과 생쥐 정강이뼈 골절에 미치는 영향 (Effects of Administration of Pyritum on Activation of Osteoblast Cells in Human Body & on Tibia Bone Fracture in Mice)

서지정보

· 발행기관 : 대한침구의학회
· 수록지 정보 : Journal of Acupuncture Research / 26권 / 2호 / 159 ~ 170페이지
· 저자명 : 황지혜, 안지현, 김진택, 안상현, 김경호, 조현석, 이승덕, 김은정, 김갑성

초록

Backgrounds and Objectives : A fracture means a loss of continuity in the substance of bone. Bone differs from other musculoskeletal tissue due to its ability to repair and heal itself without leaving a scar. The cutter head has multinucleated osteoclast cells to resorb the dead bone. The tail, with its conical surface, is lined with osteoblast cells laying down new bone. The conjugation of fracture is a unique biological process regulated by a complex array of signaling molecules and proinflammatory cytokines.
Pyritum, one of the important prescriptions in the oriental medicine, has been used for conjugation fracture. The purpose of this study is to evaluate the effects of administration of Pyritum on activation of osteoblast cells in human body & on tibia bone fracture in mice.
Materials and Methods : Four weeks aged 30 female DBA mice were used for this study. They were divided three groups, normal group, control group(fracture elicitate mice: FE group) and experimental group(Pyritum administered mice group after fracture elicitation : PA group). Left tibia bones of mice in FE and PA groups were fractured by bone cutters. MG-63 cells in human body th Pyritum in the ratio of 1 ㎎/㎖, and the cells were further incubated for 24 hours.
Activation of osteoblast was identified using osteopontin, FGF in vitro test.
In vivo test, regeneration of fractured tibia through the morphological changes was observed, and also activation of inflammation through NF-κB p65, iNOS, COX-2, osteoblast through osteopontin, FGF and osteoblast’s proliferation in each group was measured.
Results and Conclusions :
1. In vitro test for activation of osteoblast cells in human body by Pyritum, osteopontin and FGF production were remarkably increased in Pyritum treated MG-63 cells.
2. In regeneration of fractured tibia by Pyritum, fractured area in external tibia morphology was decreased more in the PA group than that of the FE group. Osteogenesis in fractured area was increased more in the PA group than that of the FE group. Also, endochodrial ossification in central area of fracture and osteoid in lateral area of fracture were increased more in the PA group than those of the FE group.
3. In activation of inflammation by Pyritum administered, activation of NF-κB p65, increase of iNOS and COX-2 production were higher in the PA and the FE groups than those of the control group. Especially, the PA group showed higher activation and increase than those of the FE group.
4. In activation of osteoblast by Pyritum, increase of osteopontin, FGF and osteoblast’s proliferation were higher in the PA and the FE groups than those of the control group. Especially, the PA group showed higher increase and proliferation than those of the FE group.

영어초록

Backgrounds and Objectives : A fracture means a loss of continuity in the substance of bone. Bone differs from other musculoskeletal tissue due to its ability to repair and heal itself without leaving a scar. The cutter head has multinucleated osteoclast cells to resorb the dead bone. The tail, with its conical surface, is lined with osteoblast cells laying down new bone. The conjugation of fracture is a unique biological process regulated by a complex array of signaling molecules and proinflammatory cytokines.
Pyritum, one of the important prescriptions in the oriental medicine, has been used for conjugation fracture. The purpose of this study is to evaluate the effects of administration of Pyritum on activation of osteoblast cells in human body & on tibia bone fracture in mice.
Materials and Methods : Four weeks aged 30 female DBA mice were used for this study. They were divided three groups, normal group, control group(fracture elicitate mice: FE group) and experimental group(Pyritum administered mice group after fracture elicitation : PA group). Left tibia bones of mice in FE and PA groups were fractured by bone cutters. MG-63 cells in human body th Pyritum in the ratio of 1 ㎎/㎖, and the cells were further incubated for 24 hours.
Activation of osteoblast was identified using osteopontin, FGF in vitro test.
In vivo test, regeneration of fractured tibia through the morphological changes was observed, and also activation of inflammation through NF-κB p65, iNOS, COX-2, osteoblast through osteopontin, FGF and osteoblast’s proliferation in each group was measured.
Results and Conclusions :
1. In vitro test for activation of osteoblast cells in human body by Pyritum, osteopontin and FGF production were remarkably increased in Pyritum treated MG-63 cells.
2. In regeneration of fractured tibia by Pyritum, fractured area in external tibia morphology was decreased more in the PA group than that of the FE group. Osteogenesis in fractured area was increased more in the PA group than that of the FE group. Also, endochodrial ossification in central area of fracture and osteoid in lateral area of fracture were increased more in the PA group than those of the FE group.
3. In activation of inflammation by Pyritum administered, activation of NF-κB p65, increase of iNOS and COX-2 production were higher in the PA and the FE groups than those of the control group. Especially, the PA group showed higher activation and increase than those of the FE group.
4. In activation of osteoblast by Pyritum, increase of osteopontin, FGF and osteoblast’s proliferation were higher in the PA and the FE groups than those of the control group. Especially, the PA group showed higher increase and proliferation than those of the FE group.

참고자료

· 없음