병아리 내이 유모세포에서 전압의존성 칼슘전류의 분리 측정 (Isolation of Voltage Dependent Calcium Current in Chick Inner Hair Cell)

서지정보

· 발행기관 : 대한이비인후과학회
· 수록지 정보 : 대한이비인후-두경부외과학회지 / 52권 / 8호 / 655 ~ 659페이지
· 저자명 : 이승환, 신명철, 안태환, 김기용, 김국, 민현정, 박철원

초록

Background and Objectives Voltage dependent calcium channel (VDCC) mediates calcium
ion influx and controls neurotransmitter release in excitable cells. Hair cells in vertebrates cochlea
are known to express L-type VDCC. The purpose of this study was to measure calcium
current from hair cells to investigate basic activity and characteristics of VDCC.
Materials and Method We measured calcium current in hair cells of the chicken’s auditory
organ, the basilar papilla analogous to the mammalian cochlea, in whose L-type, dihydropyridinesensitive
calcium channels predominate and in vestibular hair cells from cristae. Calcium current
through VDCC was isolated in voltage-clamp recording using Cesium, Tetraethylammonium, 4-
aminopyridine and apamin to block the much larger potassium currents. Various concentrations
of internal calcium buffer, ethylene glycol tetraacetic acid (EGTA) or 1,2-bis (o-aminophenoxy)
ethane-N,N,N’,N’-tetraacetic acid (BAPTA) were used.
Results The higher the buffer concentration, the larger the current size were; they were significantly
larger in 10 mM of calcium buffer concentration (ANOVA, p<0.05). There was no difference
in calcium current between cochlear and vestibular hair cells.
Conclusion We could successfully isolate stable inward calcium current from chick hair cells.
This experiment can be used as a basic method to understand neurotransmission process between
hair cells and afferent neurons.

영어초록

Background and Objectives Voltage dependent calcium channel (VDCC) mediates calcium
ion influx and controls neurotransmitter release in excitable cells. Hair cells in vertebrates cochlea
are known to express L-type VDCC. The purpose of this study was to measure calcium
current from hair cells to investigate basic activity and characteristics of VDCC.
Materials and Method We measured calcium current in hair cells of the chicken’s auditory
organ, the basilar papilla analogous to the mammalian cochlea, in whose L-type, dihydropyridinesensitive
calcium channels predominate and in vestibular hair cells from cristae. Calcium current
through VDCC was isolated in voltage-clamp recording using Cesium, Tetraethylammonium, 4-
aminopyridine and apamin to block the much larger potassium currents. Various concentrations
of internal calcium buffer, ethylene glycol tetraacetic acid (EGTA) or 1,2-bis (o-aminophenoxy)
ethane-N,N,N’,N’-tetraacetic acid (BAPTA) were used.
Results The higher the buffer concentration, the larger the current size were; they were significantly
larger in 10 mM of calcium buffer concentration (ANOVA, p<0.05). There was no difference
in calcium current between cochlear and vestibular hair cells.
Conclusion We could successfully isolate stable inward calcium current from chick hair cells.
This experiment can be used as a basic method to understand neurotransmission process between
hair cells and afferent neurons.

참고자료

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