두충의 건조법별 지표성분 및 항염증 활성 비교 (Comparison of Index Compound Content and Anti-inflammatory Activity of Eucommiae Cortex by Various Drying Methods)

서지정보

· 발행기관 : 한방재활의학과학회
· 수록지 정보 : 한방재활의학과학회지 / 27권 / 1호 / 19 ~ 25페이지
· 저자명 : 도호정, 이진호, 하인혁, 정화진, 이재웅, 김민정, 김은지, 이인희

초록

ObjectivesEucommiae cortex is one of the frequently used herbs for musculoskeletal disorders, with well-known anti-inflammatory effect. Meanwhile, powdered form of herbal drugs is more advantageous than decoction form, in that storing and taking is more convenient. Thus, the authors aimed to investigate whether the drying method affects the index compound level and anti-inflammatory effect of eucommiae cortex water extract.MethodsEucommiae cortex was extracted in distilled water at 100oC for 3 hours, filtered, and then evaporated under vacuum. One half of the sample was freeze-dried at −80oC. Another half of the sample was added with dextrin and then spray-dried at 180oC. To as-sess the possible change in index compound content, pinoresinol diglucoside was selected as the index marker. The content level of the index compound in various extract sample was quantified through high performance liquid chromatography. In addition, iNOS assay in LPS-induced RAW 264.7 cells was adopted to determine the anti-inflammatory effect of various extract samples. Furthermore, MTT assay was performed to confirm that the result of iNOS assay was not due to cytotoxicity.ResultsThere was no significance difference in index compound content between ex-tract samples obtained through two different drying methods. Anti-inflammatory activity of extract samples were similar at the matching concentration, regardless of the drying methods. Extract samples did not show any significant cytotoxicity.ConclusionsExtract samples of eucommiae cortex were obtained through freeze-drying and spray-drying. Neither change in index compound content nor difference in anti-in-flammatory activity was observed between drying methods. (J Korean Med Rehabil 2017;27(1):19-25)

영어초록

ObjectivesEucommiae cortex is one of the frequently used herbs for musculoskeletal disorders, with well-known anti-inflammatory effect. Meanwhile, powdered form of herbal drugs is more advantageous than decoction form, in that storing and taking is more convenient. Thus, the authors aimed to investigate whether the drying method affects the index compound level and anti-inflammatory effect of eucommiae cortex water extract.MethodsEucommiae cortex was extracted in distilled water at 100oC for 3 hours, filtered, and then evaporated under vacuum. One half of the sample was freeze-dried at −80oC. Another half of the sample was added with dextrin and then spray-dried at 180oC. To as-sess the possible change in index compound content, pinoresinol diglucoside was selected as the index marker. The content level of the index compound in various extract sample was quantified through high performance liquid chromatography. In addition, iNOS assay in LPS-induced RAW 264.7 cells was adopted to determine the anti-inflammatory effect of various extract samples. Furthermore, MTT assay was performed to confirm that the result of iNOS assay was not due to cytotoxicity.ResultsThere was no significance difference in index compound content between ex-tract samples obtained through two different drying methods. Anti-inflammatory activity of extract samples were similar at the matching concentration, regardless of the drying methods. Extract samples did not show any significant cytotoxicity.ConclusionsExtract samples of eucommiae cortex were obtained through freeze-drying and spray-drying. Neither change in index compound content nor difference in anti-in-flammatory activity was observed between drying methods. (J Korean Med Rehabil 2017;27(1):19-25)

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