대사증후군 백서모델에서 음경해면체의 발기능 및 조직학적 변화 (The Changes of the Erectile Function and Histology of the Corpus Cavernosum in a Rat Model of Metabolic Syndrome)

서지정보

· 발행기관 : 대한남성과학회
· 수록지 정보 : The World Journal of Men's Health / 26권 / 4호 / 187 ~ 196페이지
· 저자명 : 김성대, 조수연, 손동완, 이동섭, 정문수, 김두배, 김효신, 조용현, 박철휘, 김세웅

초록

Purpose: We evaluated the changes of the erectile function and histology of the corpus cavernosum in
a rat model of metabolic syndrome.
Materials and Methods: We used male spontaneous hypertensive rats (SHRs) as an experimental group
(n=6) and Wistar-Kyoto rats as a control group (n=6). The SHRs were fed with a high fat diet, but the
Wistar-Kyoto rats were fed with a normal fat diet for 12 weeks. All the groups were then checked for
body weight and various biochemiclal parameters. To investigate penile erection, the intracavernosal pressure
(ICP), mean arterial pressure (MAP) and cGMP level of the corpus cavernosum were recorded for all
the groups. Serial sections of the penis were used to perform Masson's trichrome staining and immunohistochemistry
for determining the TGF-β1 expression.
Results: We confirmed that metabolic syndrome was induced in the experimental group by the significant
difference of the various biochemical parameters. (ED note: For the results of erectile function?
This wasn’t clear.)As a result of erectile function, the ICP/MAP ratios were checked as 51.0±7.5% and
31.0±5.5%, respectively, for the control and experimental groups. So the ICP/MAP ratio of the latter was
markedly decreased compared with the former and the cGMP level of the corpus cavernosum was the
same for both groups. On Masson's trichrome staining, the number of smooth muscle cell was decreased
and the collagen fibers with an irregular, distorted arrangement were increased in the experimental group.
The immunoreactivity for TGF-β1 tended to increase in the experimental group. These histological findings
revealed that fibrosis of the corpus cavernosum occurred in the experimental group.
Conclusions: Our results demonstrate that metabolic syndrome is harmful to erectile function and it
leads to histological changes of the corpus cavernosum according to a rat model of metabolic syndrome

영어초록

Purpose: We evaluated the changes of the erectile function and histology of the corpus cavernosum in
a rat model of metabolic syndrome.
Materials and Methods: We used male spontaneous hypertensive rats (SHRs) as an experimental group
(n=6) and Wistar-Kyoto rats as a control group (n=6). The SHRs were fed with a high fat diet, but the
Wistar-Kyoto rats were fed with a normal fat diet for 12 weeks. All the groups were then checked for
body weight and various biochemiclal parameters. To investigate penile erection, the intracavernosal pressure
(ICP), mean arterial pressure (MAP) and cGMP level of the corpus cavernosum were recorded for all
the groups. Serial sections of the penis were used to perform Masson's trichrome staining and immunohistochemistry
for determining the TGF-β1 expression.
Results: We confirmed that metabolic syndrome was induced in the experimental group by the significant
difference of the various biochemical parameters. (ED note: For the results of erectile function?
This wasn’t clear.)As a result of erectile function, the ICP/MAP ratios were checked as 51.0±7.5% and
31.0±5.5%, respectively, for the control and experimental groups. So the ICP/MAP ratio of the latter was
markedly decreased compared with the former and the cGMP level of the corpus cavernosum was the
same for both groups. On Masson's trichrome staining, the number of smooth muscle cell was decreased
and the collagen fibers with an irregular, distorted arrangement were increased in the experimental group.
The immunoreactivity for TGF-β1 tended to increase in the experimental group. These histological findings
revealed that fibrosis of the corpus cavernosum occurred in the experimental group.
Conclusions: Our results demonstrate that metabolic syndrome is harmful to erectile function and it
leads to histological changes of the corpus cavernosum according to a rat model of metabolic syndrome

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