Improving the Survival and Maintenance of the Undifferentiated State of Cryopreserved Human Embryonic Stem Cells by Extended Incubation with Feeder Cells Overnight before Vitrification

서지정보

· 발행기관 : 한국발생생물학회
· 수록지 정보 : 발생과 생식 / 12권 / 2호 / 141 ~ 149페이지
· 저자명 : 차수경, 최경희, 신주미, 박규형, 윤태기, 정형민, 이동율

초록

This study was conducted to develop an efficient cryopreservation method of human embryonic stem (ES) cells using vitrification. In an initial experiment, sub-clumps of human ES cells (CHA-hES3 and CHA-hES4) were vitrified using grids after incubation with STO feeder cells for 1 or 16 h (Groups 1-1 and 1-2, respectively). After storage for 2-4 months, thawed clumps were re-plated on a fresh feeder layer. The survival rates of warmed CHA-hES3 and CHA-hES4 cells of Group 1-2 were significantly higher than those of the corresponding Group 1-1 cells. In the second experiment, human ES cells were vitrified after incubation with feeder or feeder-conditioned medium (Groups 2-1 to -7). Relative mRNA expression of BM proteins and survival rates were increased following incubation of ES cells with fresh feeder cells for 16 h. In conclusion, increasing of tight adhesion between ES cells by extended incubation with feeder could reduce cryoinjury after vitrifying/warming.

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