톳 메탄올 추출물에 의한 인체 폐암 세포의 Apoptosis 유발 (Apoptosis Induction of Human Lung Cancer Cells by Methyl Alcohol Extracts of Hizikia fusiforme)

서지정보

· 발행기관 : 대한암예방학회
· 수록지 정보 : 대한암예방학회지 / 15권 / 2호 / 164 ~ 171페이지
· 저자명 : 박신형, 신동역, 엄현섭, 지규용, 최영현

초록

Hizikia fusiforme, a brown alga, has been reported to possess a wide range of beneficial activities including immuno-modulating, anti-oxidants, anti-coagulants, and anti-carcinogenic effects. According to recent studies, H. fusiforme can inhibit the growth of cancer cell by inducing apoptosis in a certain cancer lines in vitro. However, the molecular mechanism how it acts as an inducer of apoptosis is not fully understood. In this study, we investigated the apoptotic effects of H. fusiforme methyl alcohol extracts (MEHF) in A549 and NCI-H460 human lung carcinoma cell lines. It was shown that MEHF induced the cell growth inhibition in a dose-dependant manner and it was more significant in NCI-H460 cells.
There also appeared increased sub-G1 phase DNA content, chromatin condensation, as well as DNA fragmentation in MEHF-treated cells, which are indicators of apoptosis. MEHF activated the caspase-8via up-regulating Fas, FasL and DR5 expression and cleavage of Bid protein. MEHF also up-regulated the pro-apoptotic Bax levels and down-regulated anti-apoptotic Bcl-xL expression, which was associated with the loss of mitochondrial membrane potential (MMP) and activation of caspase-9 and caspase-3.
Taken together, these findings suggest that H. fusiforme might be useful for clinical application to treat human lung cancer. (Cancer Prev Res 15, 164-171, 2010)

영어초록

Hizikia fusiforme, a brown alga, has been reported to possess a wide range of beneficial activities including immuno-modulating, anti-oxidants, anti-coagulants, and anti-carcinogenic effects. According to recent studies, H. fusiforme can inhibit the growth of cancer cell by inducing apoptosis in a certain cancer lines in vitro. However, the molecular mechanism how it acts as an inducer of apoptosis is not fully understood. In this study, we investigated the apoptotic effects of H. fusiforme methyl alcohol extracts (MEHF) in A549 and NCI-H460 human lung carcinoma cell lines. It was shown that MEHF induced the cell growth inhibition in a dose-dependant manner and it was more significant in NCI-H460 cells.
There also appeared increased sub-G1 phase DNA content, chromatin condensation, as well as DNA fragmentation in MEHF-treated cells, which are indicators of apoptosis. MEHF activated the caspase-8via up-regulating Fas, FasL and DR5 expression and cleavage of Bid protein. MEHF also up-regulated the pro-apoptotic Bax levels and down-regulated anti-apoptotic Bcl-xL expression, which was associated with the loss of mitochondrial membrane potential (MMP) and activation of caspase-9 and caspase-3.
Taken together, these findings suggest that H. fusiforme might be useful for clinical application to treat human lung cancer. (Cancer Prev Res 15, 164-171, 2010)

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