연잎 에탄올 추출물이 피부 주름에 미치는 영향 (Effects of ethanol extract of Nelumbo nucifera leaves on Anti-oxidation and Type I Procollagen Expression in CCD-986sk Cells)

서지정보

· 발행기관 : 대한한의학방제학회
· 수록지 정보 : 대한한의학 방제학회지 / 14권 / 2호 / 67 ~ 75페이지
· 저자명 : 양웅모, 김윤경, 박성규, 김향미, 장문석, 박완수, 김원남, 김산웅, 최동기, 이학철

초록

The natural plants were screened for new cosmetic materials. Many parts of Nelumbo nucifera has been investigated for anti-wrinkling and anti-furrowing effects. The leaves, flowers and stamens of Nelumbo nucifera were extracted with ethanol or distilled water. The effects of cell viability and H2O2-induced cytotoxicity were studied on CCD-986sk human fibroblast cell line by MTT assay. We measured various effects related to skin such as the synthesis of MMP-1 and type I procollagen. The ethanol extract of Nelumbo nucifera leaves (ENL) at concentrations of 30 μg/ml showed maximum cell viability on CCD-986sk cells by 129.70%, and H2O2-induced cytotoxicity was blocked by ENL (5, 10, 20, 30, and 40 μg/ml) concentration-dependently. The synthesis of MMP-1 and type I procollagen were positive in ENL. These results suggest that ENL has anti-oxidant and anti-furrowing effects.
Key words: Nelumbo nucifera, CCD-986sk human fibroblast cell line, ethanol extract of Nelumbo nucifera leaves (ENL), MMP-1, type I procollagen

영어초록

The natural plants were screened for new cosmetic materials. Many parts of Nelumbo nucifera has been investigated for anti-wrinkling and anti-furrowing effects. The leaves, flowers and stamens of Nelumbo nucifera were extracted with ethanol or distilled water. The effects of cell viability and H2O2-induced cytotoxicity were studied on CCD-986sk human fibroblast cell line by MTT assay. We measured various effects related to skin such as the synthesis of MMP-1 and type I procollagen. The ethanol extract of Nelumbo nucifera leaves (ENL) at concentrations of 30 μg/ml showed maximum cell viability on CCD-986sk cells by 129.70%, and H2O2-induced cytotoxicity was blocked by ENL (5, 10, 20, 30, and 40 μg/ml) concentration-dependently. The synthesis of MMP-1 and type I procollagen were positive in ENL. These results suggest that ENL has anti-oxidant and anti-furrowing effects.
Key words: Nelumbo nucifera, CCD-986sk human fibroblast cell line, ethanol extract of Nelumbo nucifera leaves (ENL), MMP-1, type I procollagen

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