오레가노 잎 추출물에 대한 항염증 및 미백 활성 (Anti-inflammatory and Whitening Activity of Origanum vulgare Leaves Extract)

서지정보

· 발행기관 : 대한미용학회
· 수록지 정보 : 대한미용학회지 / 19권 / 3호 / 149 ~ 156페이지
· 저자명 : 유단희, 이인철

초록

In this study, the anti-inflammatory and whitening effects of hot water and 70% ethanol extracts of Origanum vulgare leaf were examined. First, the results from measurement of the total polyphenol content confirmed that OLD was 649.16 ±0.41 mg/100 g and OLE was 646.62 ±0.85 mg/100 g. Analysis of electron donating activity and ABTS radical scavenging activity was performed for measurement of the antioxidant activity, which confirmed that both extracts exerted antioxidant activity in a concentration-dependent manner. The results of measurement of tyrosinase inhibition activity showed an inhibitory effect of 41.52% for OLE at a concentration of 1,000 μg/mL. Assessment of cytotoxicity in RAW 264.7 cells and B16-F10 cells was then performed for measurement of anti-inflammatory and whitening effects at the cellular level. RAW 264.7 cells showed cell viability of more than 95% at a concentration of 500 μg/mL, and B16-F10 cells showed cell viability of more than 95% at a concentration of 50 μg/mL. Measurement of the inhibitory activity on production of NO was performed in order to verify anti-inflammatory activity. The results confirmed that production of NO was decreased by both OLD and OLE in a concentration-dependent manner compared to the group treated with LPS alone. The inhibitory activity on melanin synthesis was determined for evaluation of the whitening effect. The results indicated that melanin synthesis was inhibited by both OLD and OLE in a concentration-dependent manner compared to the group treated with α-MSH alone. In conclusion, the results confirmed the antioxidant, anti-inflammatory, and whitening effects of OLD and OLE, indicating the potential for their use as new natural materials.

영어초록

In this study, the anti-inflammatory and whitening effects of hot water and 70% ethanol extracts of Origanum vulgare leaf were examined. First, the results from measurement of the total polyphenol content confirmed that OLD was 649.16 ±0.41 mg/100 g and OLE was 646.62 ±0.85 mg/100 g. Analysis of electron donating activity and ABTS radical scavenging activity was performed for measurement of the antioxidant activity, which confirmed that both extracts exerted antioxidant activity in a concentration-dependent manner. The results of measurement of tyrosinase inhibition activity showed an inhibitory effect of 41.52% for OLE at a concentration of 1,000 μg/mL. Assessment of cytotoxicity in RAW 264.7 cells and B16-F10 cells was then performed for measurement of anti-inflammatory and whitening effects at the cellular level. RAW 264.7 cells showed cell viability of more than 95% at a concentration of 500 μg/mL, and B16-F10 cells showed cell viability of more than 95% at a concentration of 50 μg/mL. Measurement of the inhibitory activity on production of NO was performed in order to verify anti-inflammatory activity. The results confirmed that production of NO was decreased by both OLD and OLE in a concentration-dependent manner compared to the group treated with LPS alone. The inhibitory activity on melanin synthesis was determined for evaluation of the whitening effect. The results indicated that melanin synthesis was inhibited by both OLD and OLE in a concentration-dependent manner compared to the group treated with α-MSH alone. In conclusion, the results confirmed the antioxidant, anti-inflammatory, and whitening effects of OLD and OLE, indicating the potential for their use as new natural materials.

참고자료

· 없음