金銀花 열수 추출물이 피부 美白에 미치는 영향 (Effects of Hot Water Extracts from Lonicera Japonica Flos Extracts on whitening using B16F10 cell lines)

서지정보

· 발행기관 : 대한한방안이비인후피부과학회
· 수록지 정보 : 한방안이비인후피부과학회지 / 30권 / 2호 / 38 ~ 50페이지
· 저자명 : 우배언, 김혜화, 이유림, 박수연, 정민영, 최정화

초록

Objectives : This study was designed to investigate effects of Lonicera Flos Extracts(LFE) on whitening using B16F10 cell lines.
Methods : In this experiment, we observed effects of LFE on cell viability, inhibitory effects of melanin synthesis, inhibitory effect on tyrosinase, tyrosinase activity, superoxide dismutase(SOD)-like activity and mRNA expression.
Results : In results, more than 2000㎍/㎖ of LFE treated group showed lowered cell viability rates significantly compared to albutin treated group. More than 2000㎍/㎖ of LFE treated groups were lower levels of melanin synthesis. Inhibitory effects of melanin production showed in 1000㎍/㎖ of LFE treated group. 1,000㎍/㎖ of LFE treated group significantly suppressed tyrosinase activities in vitro. LFE and albutin treated group significantly decreased tyrosinase activity compared to non treated group. SOD-like activity of LFE treated group was lower than vitamin C treated group but increased depending on concentration. 500㎍/㎖ of LFE treated group and 1,000 ㎍/㎖ of LFE treated group was significantly increased. Tyrosinase mRNA expression of α-MSH and LFE 250㎍/㎖ treated group significantly decreased compared to α-MSH treated group.
Conclusions : These results suggest that LFE can inhibit melanin synthesis through inhibitory action on tyrosinase activity. And LFE suppressed tyrosinase activities B16F10 cells significantly. So I suggest LFE can apply to whitening

영어초록

Objectives : This study was designed to investigate effects of Lonicera Flos Extracts(LFE) on whitening using B16F10 cell lines.
Methods : In this experiment, we observed effects of LFE on cell viability, inhibitory effects of melanin synthesis, inhibitory effect on tyrosinase, tyrosinase activity, superoxide dismutase(SOD)-like activity and mRNA expression.
Results : In results, more than 2000㎍/㎖ of LFE treated group showed lowered cell viability rates significantly compared to albutin treated group. More than 2000㎍/㎖ of LFE treated groups were lower levels of melanin synthesis. Inhibitory effects of melanin production showed in 1000㎍/㎖ of LFE treated group. 1,000㎍/㎖ of LFE treated group significantly suppressed tyrosinase activities in vitro. LFE and albutin treated group significantly decreased tyrosinase activity compared to non treated group. SOD-like activity of LFE treated group was lower than vitamin C treated group but increased depending on concentration. 500㎍/㎖ of LFE treated group and 1,000 ㎍/㎖ of LFE treated group was significantly increased. Tyrosinase mRNA expression of α-MSH and LFE 250㎍/㎖ treated group significantly decreased compared to α-MSH treated group.
Conclusions : These results suggest that LFE can inhibit melanin synthesis through inhibitory action on tyrosinase activity. And LFE suppressed tyrosinase activities B16F10 cells significantly. So I suggest LFE can apply to whitening

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