약용버섯 β-glucan 추출물과 정제물의 특성과 생리활성 (Characterization of β-glucan extracts and purified extracts from medicinal mushrooms and their biological activities)

서지정보

· 발행기관 : 한국식품저장유통학회
· 수록지 정보 : Food Science and Preservation / 26권 / 3호 / 315 ~ 321페이지
· 저자명 : 김하나, 김주영, 김영찬, 김창섭, 정영훈, 정신교

초록

상황버섯과 영지버섯의 β-glucan을 viscozyme L 처리하여 추출하고 정제하여 이화학적인 특성과 항산화활성, 암세포생육억제활성을 조사하였다. β-Glucan의 적정 추출 조건은 Viscozyme L 반응시간 8시간, 기질에 대한 효소 농도 6%(v/w), 상황버섯과 영지버섯의 혼합 비율 50:50(w/w)이었다. 정제물인 PGBEP(40.19%)의 β-glucan 순도는 추출물인 PGBE(26.70%)에 비하여 약 1.5배 높았으나, 추출 수율이 약 3배 낮아, 이를 고려한 β-glucan 함량은 추출물인 PGBE(5.23 g/100 g)가 정제물인 PGBEP(2.57 g/100 g)에 비하여 약 2배 높았다. 적외선흡수스펙트럼에서 β-glucan의 α-glycosidic linkage와 β-glycosidic linkage를 확인하였다. 항산화성분 함량과 항산화 활성은 추출물인 PGBE가 정제물인 PGBEP에 비하여 더 높게 나타났다. MTT assay에 의한 세포독성은 PGBE와 PGBEP 모두 나타나지 않았다. Wound healing assay로 측정한 암세포생육억제활성은 정제물인 PGBEP가 추출물인 PGBE보다 높았으며, 양성대조구인 resveratrol(10 μg/mL)보다 높았다(p<0.05).

영어초록

Phellinus baumii and Ganoderma lucidum are the representative medicinal mushrooms in Korea. The β-glucan extract was prepared from these mushrooms, and their physicochemical characteristics, antioxidant contents, antioxidant activities, cell viability, and inhibition activities of cancer cell migration were investigated. The optimum β-glucan extraction condition was 6% (v/w) concentration of enzyme (Viscozyme L), 50:50 (w/w) mushroom ratio, and 8 hours of reaction time, respectively. The β-glucan purity of PGBEP (Phellinus baumii and Ganoderma lucidum β-glucan extract with purification) was higher than of PGBE (Phellinus baumii and Ganoderma lucidum β-glucan extract). In contrast, the extraction yield and β-glucan contents of PGBEP were lower than of PGBE (p<0.05). The FT-IR spectrum of PGBE and PGBEP showed that α-glycosidic and β-glycosidic linkage formation was determined by the appearance of the peaks at 890-822 cm-1 range. The antioxidant contents and antioxidant activities of PGBE were higher than of PGBEP. The cell viability by MTT assay of PGBE and PGBEP was higher than 80%. The inhibition activity of cancer cell migration by wound healing assay of PGBEP was significantly higher than of PGBE and positive control.

참고자료

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