방석나물 추출물과 그 분획물의 항산화, 항염 및 피부 보호 효능 (Anti-oxidant, anti-inflammatory, and skin protective effects of the crude extract and its fractions from Suaeda australis)

서지정보

· 발행기관 : 국제보건미용학회
· 수록지 정보 : 국제보건미용학회지 / 17권 / 2호 / 82 ~ 94페이지
· 저자명 : 박지효, 이지안

초록

This study aims to assess the anti-oxidant, anti-inflammatory, and anti-microbial effects of Suaeda australis, which is known as halophytes. Methanol extracts of S. australis were fractioned according to the polarity with n-hexane, chloroform, ethyl acetate, n-butanol, and H2O. The anti-oxide activity of the fractions was evaluated using DPPH radical scavenging activity and total polyphenol content. All solvent fractions showed potent anti-oxidative effects, as assessed using radical scavenging activity, with IC50 values ranging from 109.782 to 754.691 ㎍/mL. In agreement with this pattern, the total phenolic contents were widely distributed in solvent fractions, and ranged from 10.746 to 50.672 mg/g GAE of dry weight. In the evaluation of anti-inflammatory effects, LPS-activated RAW264.7 macrophages suppressed the expression of iNOS and COX-2, as well as the production of TNF-α and IL-6. Skin barrier protective effects were investigated in HaCaT cells measuring the mRNA expression of skin barrier integrity and hydration genes, namely, HBD (human β-defensin), LL-37(cathelicidin), and HAS (hyaluronic acid synthase). The ethyl acetate fraction showed the strongest skin barrier integrity. Based on the current results, S. australis was suggested to posses natural anti-oxidant and anti-inflammatory agents, and skin protective materials, indicating that it could be a valuable source of multi-functional cosmetic ingredients.

영어초록

This study aims to assess the anti-oxidant, anti-inflammatory, and anti-microbial effects of Suaeda australis, which is known as halophytes. Methanol extracts of S. australis were fractioned according to the polarity with n-hexane, chloroform, ethyl acetate, n-butanol, and H2O. The anti-oxide activity of the fractions was evaluated using DPPH radical scavenging activity and total polyphenol content. All solvent fractions showed potent anti-oxidative effects, as assessed using radical scavenging activity, with IC50 values ranging from 109.782 to 754.691 ㎍/mL. In agreement with this pattern, the total phenolic contents were widely distributed in solvent fractions, and ranged from 10.746 to 50.672 mg/g GAE of dry weight. In the evaluation of anti-inflammatory effects, LPS-activated RAW264.7 macrophages suppressed the expression of iNOS and COX-2, as well as the production of TNF-α and IL-6. Skin barrier protective effects were investigated in HaCaT cells measuring the mRNA expression of skin barrier integrity and hydration genes, namely, HBD (human β-defensin), LL-37(cathelicidin), and HAS (hyaluronic acid synthase). The ethyl acetate fraction showed the strongest skin barrier integrity. Based on the current results, S. australis was suggested to posses natural anti-oxidant and anti-inflammatory agents, and skin protective materials, indicating that it could be a valuable source of multi-functional cosmetic ingredients.

참고자료

· 없음