가토의 귀에서 무세포성 진피 기질의 이식 방법에 따른 치유 양상 (HEALING PATTERNS OF THE ACELLULAR DERMAL MATRIX DEPEND ON GRAFT METHOD IN THE RABBIT EARS)

서지정보

· 발행기관 : 대한악안면성형재건외과학회
· 수록지 정보 : Maxillofacial Plastic Reconstructive Surgery / 31권 / 3호 / 216 ~ 221페이지
· 저자명 : 류재영, 유선열

초록

Purpose: The retention of the basement membrane complex, which was the unique feature of the acellular
dermal matrix (AlloDerm®), plays an important role in the normal process of wound healing. The present
study was aimed to compare the healing of the acellular dermal matrix according to the graft method
in the rabbit ear.
Materials and methods: Six mature rabbits weighing about 3.0 kg were used, 10 × 5 mm sized subcutaneous
pockets were created between the ear skin and the underlying perichondrium. In the control
group, the acellular dermal matrix was grafted with the basement membrane facing toward the perichondrium.
On the contrary, the acellular dermal matrix was grafted with the basement membrane facing
toward the skin side in the experimental group Ⅰ. In the experimental group Ⅱ, the acellular dermal
matrix was grafted like rolled configuration with basement membrane side in. The grafted site was picked
at 3, 7, and 21 days after the graft. Serial sections were processed by H-E stain and examined under light
microscopy to assess the healing patterns.
Results: There was no distinct volume loss in the gross examination, but resorption was observed from
the edge of the acellular dermal matrix in the histological examination. The space of resorption was
replaced by the newly formed fibrous tissues and vessels. The inflammatory cells were more increased at 7
days after the graft than the early days. However, inflammation was decreased at 21 days after the graft.
Regardless of the graft direction, no differences were observed between the control and the experimental
group Ⅰ in the healing patterns.
Conclusion: These results suggest that the acellular dermal matrix can be used simply and effectively
without regard to the graft direction as a substitute of autogenous material for repairing soft tissue defect.

영어초록

Purpose: The retention of the basement membrane complex, which was the unique feature of the acellular
dermal matrix (AlloDerm®), plays an important role in the normal process of wound healing. The present
study was aimed to compare the healing of the acellular dermal matrix according to the graft method
in the rabbit ear.
Materials and methods: Six mature rabbits weighing about 3.0 kg were used, 10 × 5 mm sized subcutaneous
pockets were created between the ear skin and the underlying perichondrium. In the control
group, the acellular dermal matrix was grafted with the basement membrane facing toward the perichondrium.
On the contrary, the acellular dermal matrix was grafted with the basement membrane facing
toward the skin side in the experimental group Ⅰ. In the experimental group Ⅱ, the acellular dermal
matrix was grafted like rolled configuration with basement membrane side in. The grafted site was picked
at 3, 7, and 21 days after the graft. Serial sections were processed by H-E stain and examined under light
microscopy to assess the healing patterns.
Results: There was no distinct volume loss in the gross examination, but resorption was observed from
the edge of the acellular dermal matrix in the histological examination. The space of resorption was
replaced by the newly formed fibrous tissues and vessels. The inflammatory cells were more increased at 7
days after the graft than the early days. However, inflammation was decreased at 21 days after the graft.
Regardless of the graft direction, no differences were observed between the control and the experimental
group Ⅰ in the healing patterns.
Conclusion: These results suggest that the acellular dermal matrix can be used simply and effectively
without regard to the graft direction as a substitute of autogenous material for repairing soft tissue defect.

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