가토에서 조직 재생 이식재로서 무세포성 진피 기질의 효용 (Usefulness of acellular dermal matrix graft on the tissue regeneration in rabbits)

서지정보

· 발행기관 : 대한구강악안면외과학회
· 수록지 정보 : 대한구강악안면외과학회지 / 34권 / 2호 / 220 ~ 229페이지
· 저자명 : 최종학, 류재영, 유선열

초록

The present study was aimed to examine the effect of acellular dermal matrix (AlloDerm ) grafted to the experimental tissue defect on tissue
regeneration.
Materials and Methods: Male albino rabbits were used. Soft tissue defects were prepared in the external abdominal oblique muscle. The animals
were then divided into 3 groups by the graft material used: no graft, autogenous dermis graft, and AlloDerm graft. The healing sites were histologically
examined at weeks 4 and 8 after the graft. In another series, critical sized defects with 8-㎜ diameter were prepared in the right and left iliac
bones. The animals were then divided into 5 groups: no graft, grafted with autogenous iliac bone, AlloDerm graft, AlloDerm graft impregnated with
rhBMP-2, and AlloDerm graft with rhTGF- 1. The healing sites of bone defect were investigated with radiologic densitometry and histological
evaluation at weeks 4 and 8 after the graft.
Results: In the soft tissue defect, normal healing was seen in the group of no graft. Inflammatory cells and foreign body reactions were observed in
the group of autogenous dermis graft, and the migration of fibroblasts and the formation of vessels into the collagen fibers were observed in the group
of AlloDerm graft. In the bone defect, the site of bone defect was healed by fibrous tissues in the group of no graft. The marked radiopacity and good
regeneration were seen in the group of autogenous bone graft. There remained the traces of AlloDerm with no satisfactory results in the group of
AlloDerm graft. In the groups of the AlloDerm graft with rhBMP-2 or rhTGF- 1, there were numerous osteoblasts in the boundary of the adjacent
bone which was closely approximated to the AlloDerm with regeneration features. However, the fibrous capsule also remained as in the group of
AlloDerm graft, which separated the AlloDerm and the adjacent bone.
Conclusions: These results suggest that AlloDerm can be useful to substitute the autogenous dermis in the soft tissue defect. However, it may not
be useful as a bone graft material or a carrier, since the bone defect was not completely healed by the bony tissue, regardless of the presence of
osteogenic factors like rhBMP-2 or rhTGF- 1.

영어초록

The present study was aimed to examine the effect of acellular dermal matrix (AlloDerm ) grafted to the experimental tissue defect on tissue
regeneration.
Materials and Methods: Male albino rabbits were used. Soft tissue defects were prepared in the external abdominal oblique muscle. The animals
were then divided into 3 groups by the graft material used: no graft, autogenous dermis graft, and AlloDerm graft. The healing sites were histologically
examined at weeks 4 and 8 after the graft. In another series, critical sized defects with 8-㎜ diameter were prepared in the right and left iliac
bones. The animals were then divided into 5 groups: no graft, grafted with autogenous iliac bone, AlloDerm graft, AlloDerm graft impregnated with
rhBMP-2, and AlloDerm graft with rhTGF- 1. The healing sites of bone defect were investigated with radiologic densitometry and histological
evaluation at weeks 4 and 8 after the graft.
Results: In the soft tissue defect, normal healing was seen in the group of no graft. Inflammatory cells and foreign body reactions were observed in
the group of autogenous dermis graft, and the migration of fibroblasts and the formation of vessels into the collagen fibers were observed in the group
of AlloDerm graft. In the bone defect, the site of bone defect was healed by fibrous tissues in the group of no graft. The marked radiopacity and good
regeneration were seen in the group of autogenous bone graft. There remained the traces of AlloDerm with no satisfactory results in the group of
AlloDerm graft. In the groups of the AlloDerm graft with rhBMP-2 or rhTGF- 1, there were numerous osteoblasts in the boundary of the adjacent
bone which was closely approximated to the AlloDerm with regeneration features. However, the fibrous capsule also remained as in the group of
AlloDerm graft, which separated the AlloDerm and the adjacent bone.
Conclusions: These results suggest that AlloDerm can be useful to substitute the autogenous dermis in the soft tissue defect. However, it may not
be useful as a bone graft material or a carrier, since the bone defect was not completely healed by the bony tissue, regardless of the presence of
osteogenic factors like rhBMP-2 or rhTGF- 1.

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