분자표지를 활용한 고품질 가공용 고순도 무 품종 육성 (Development of highly uniform variety for processing using SSR markers in radish (Raphanus sativus L))

서지정보

· 발행기관 : 한국식물생명공학회
· 수록지 정보 : Journal of Plant Biotechnology / 41권 / 1호 / 56 ~ 63페이지
· 저자명 : 정운화, 이용범, 오종혁, 김영규, 안춘희, 이광식, 최수련, 임용표, 박수형, 최기영

초록

Using commercial radish varieties for processing,about 30% of radish was discarded due to the root shape andlow purity. To raise the processing ability, we tried todevelop a new variety producing H-shaped root. As anothercharacteristic required in variety for processing is highpurity, we tried to raise purity using simple sequence repeats(SSR) markers for testing seed purity in every segregatinggeneration. To develop Male-sterile (MS) seeding parent,we crossed commercial variety of ‘Gwan dong spring’ and‘Gyeo ryong spring’. One elite inbred was selected asrecurrent parent for the MS plant. The major horticulturaltraits of selected inbred line were disease resistance, latebolting, heat resistance and bright green root top color. Todevelop pollen parent, we crossed commercial variety of‘Tae sang king’ and ‘Seoul spring’. We used individualselection method to develop H-shaped hard root and diseaseresistant inbred. In each segregating generation, we selectedone plant based on phenotype and the uniformity of selectedplant was tested by SSR markers using self-pollinated seeds.
In the first segregating generation, 64.6% of sib plants sharedthe same band in PCR amplification using ACMP-490primer and 66.7% using cnu-316 primer. The uniformity ofsegregating generations using ACMP-490 and cnu-316raised in second generation to 68.8%, 70.8%, respectively;in third generation to 93.8%, 100%; in fourth generation to93.8%, 100%; in fifth generation to 95.8%, 100%; in sixthgeneration to 100%, 100%. A novel cross was made usingselected MS parent and pollen parent. When we checker thehorticultural traits using autumn cultivation, the novel crossvariety produced H-shaped root comparing other commercialvarieties and produced highly uniform radish. Thus weregistered this novel cross variety as ‘YR ORE’ at 2013(Registration No. 4550).
Keywords Radish, Processing

영어초록

Using commercial radish varieties for processing,about 30% of radish was discarded due to the root shape andlow purity. To raise the processing ability, we tried todevelop a new variety producing H-shaped root. As anothercharacteristic required in variety for processing is highpurity, we tried to raise purity using simple sequence repeats(SSR) markers for testing seed purity in every segregatinggeneration. To develop Male-sterile (MS) seeding parent,we crossed commercial variety of ‘Gwan dong spring’ and‘Gyeo ryong spring’. One elite inbred was selected asrecurrent parent for the MS plant. The major horticulturaltraits of selected inbred line were disease resistance, latebolting, heat resistance and bright green root top color. Todevelop pollen parent, we crossed commercial variety of‘Tae sang king’ and ‘Seoul spring’. We used individualselection method to develop H-shaped hard root and diseaseresistant inbred. In each segregating generation, we selectedone plant based on phenotype and the uniformity of selectedplant was tested by SSR markers using self-pollinated seeds.
In the first segregating generation, 64.6% of sib plants sharedthe same band in PCR amplification using ACMP-490primer and 66.7% using cnu-316 primer. The uniformity ofsegregating generations using ACMP-490 and cnu-316raised in second generation to 68.8%, 70.8%, respectively;in third generation to 93.8%, 100%; in fourth generation to93.8%, 100%; in fifth generation to 95.8%, 100%; in sixthgeneration to 100%, 100%. A novel cross was made usingselected MS parent and pollen parent. When we checker thehorticultural traits using autumn cultivation, the novel crossvariety produced H-shaped root comparing other commercialvarieties and produced highly uniform radish. Thus weregistered this novel cross variety as ‘YR ORE’ at 2013(Registration No. 4550).
Keywords Radish, Processing

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