인진과 황련 추출물의 췌장암 세포주 MIA PaCa-2에 대한세포사멸 효과 (Apoptotic Effect of ARTEMISIAE CAPILLARIS HERBA and COPTIDIS RHIZOMA Extracts on MIA PaCa-2 Cells)

서지정보

· 발행기관 : 대한한방안이비인후피부과학회
· 수록지 정보 : 한방안이비인후피부과학회지 / 27권 / 4호 / 158 ~ 176페이지
· 저자명 : 주현아, 배현진, 황충연

초록

Purpose : The purpose of this study is to investigate the effect of ARTEMISIAE CAPILLARIS HERBA andCOPTIDIS RHIZOMA Extracts on cell death in pancreatic cancer cells.
Method : Human-derived pancreatic cancer cell line, MIA PaCa-2 cells were treated by Prescription A withvarious concentrations and the cytotoxicity was determined by MTT assay. To investigate the effects of PrescriptionA on pancreatic cancer cells, the staining of Annexin V/PI, cell cycle arrest, nuclear chromatin condensation andthe production of reactive oxygen species (ROS) were examined. The effect of Prescription A's effectivecomponents, ARTEMISIAE CAPILLARIS HERBA and COPTIDIS RHIZOMA Extracts on cell death were alsoobserved.
Results : The viability of MIA PaCa-2 cells treated with Prescription A were decreased in a dose dependentmanner. Prescription A induced cell death in MIA PaCa-2 cells as shown by result of Annexin V/PI doublestaining, chromatin condensation and cell cycle arrest. In addition, production of ROS was increased byPrescription A treatment, suggesting that ROS induced by Prescription A mediated cell death. Furthermore,Prescription A's effective components, ARTEMISIAE CAPILLARIS HERBA and COPTIDIS RHIZOMA Extracts werealso induced apoptosis of MIA PaCa-2 cells through ROS production.
Conclusion : These results suggest that Prescription A's effective components, ARTEMISIAE CAPILLARIS HERBAand COPTIDIS RHIZOMA Extracts induced death of MIA PaCa-2 through ROS production.

영어초록

Purpose : The purpose of this study is to investigate the effect of ARTEMISIAE CAPILLARIS HERBA andCOPTIDIS RHIZOMA Extracts on cell death in pancreatic cancer cells.
Method : Human-derived pancreatic cancer cell line, MIA PaCa-2 cells were treated by Prescription A withvarious concentrations and the cytotoxicity was determined by MTT assay. To investigate the effects of PrescriptionA on pancreatic cancer cells, the staining of Annexin V/PI, cell cycle arrest, nuclear chromatin condensation andthe production of reactive oxygen species (ROS) were examined. The effect of Prescription A's effectivecomponents, ARTEMISIAE CAPILLARIS HERBA and COPTIDIS RHIZOMA Extracts on cell death were alsoobserved.
Results : The viability of MIA PaCa-2 cells treated with Prescription A were decreased in a dose dependentmanner. Prescription A induced cell death in MIA PaCa-2 cells as shown by result of Annexin V/PI doublestaining, chromatin condensation and cell cycle arrest. In addition, production of ROS was increased byPrescription A treatment, suggesting that ROS induced by Prescription A mediated cell death. Furthermore,Prescription A's effective components, ARTEMISIAE CAPILLARIS HERBA and COPTIDIS RHIZOMA Extracts werealso induced apoptosis of MIA PaCa-2 cells through ROS production.
Conclusion : These results suggest that Prescription A's effective components, ARTEMISIAE CAPILLARIS HERBAand COPTIDIS RHIZOMA Extracts induced death of MIA PaCa-2 through ROS production.

참고자료

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