Prevalence of Methicillin-resistant Staphylococci Isolates from Horses and Horse-related Personnel in Korea

서지정보

· 발행기관 : 한국임상수의학회
· 수록지 정보 : 한국임상수의학회지 / 31권 / 3호 / 194 ~ 198페이지
· 저자명 : 이상규, 한재익, 김일화, 나기정, 강현구

초록

Methicillin-resistant staphylococci (MRS) are emerging as important pathogens in humans and animalsworldwide. The aim of this study was to investigate the prevalence of MRS in the racehorse population and in horserelatedpersonnel in Korea. A total of 195 horses and 18 humans (eight veterinarians, three veterinary hospital staff,and seven horse-handlers) from racehorse farms in Korea were included in the study. The samples were collectedfrom nasal cavities using bacterial transport medium and were cultivated on tryptic soy agar with 5% sheep bloodfor 3 days at 37oC to confirm the presence of Staphylococcus spp. Presumptive Staphylococcus spp. isolates wereidentified by 16S ribosomal RNA gene analysis. The coagulase test and oxacillin susceptibility tests were performedusing the tube dilution and disk diffusion methods, respectively. The presence of the mecA gene was determined usinga polymerase chain reaction assay. Of the 195 horses, 29 (15.6%) yielded 29 MRS isolates. Twelve (66.7%) of the18 horse-related personnel yielded 12 MRS isolates. All of the MRS isolates from horses or horse-related personnelwere identified as methicillin-resistant coagulase-negative staphylococci (MRCNS). The result of this study suggestthat the prevalence of MRS increased with the duration of antibiotic use (p = 0.002). This study also provides evidencefor the zoonotic transmission of MRCNS between horses and humans, although further investigations are needed.

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