백서의 횡격막에서 Succinylcholine 제이상 차단과 약역학 (Succinylcholine-induced phase II block and pharmacodynamics in rat diaphragm)

서지정보

· 발행기관 : 대한마취통증의학회
· 수록지 정보 : Anesthesia and Pain Medicine / 3권 / 4호 / 304 ~ 308페이지
· 저자명 : 이승철

초록

Background: Succinylcholine causes phase II block by a large
dose or a prolonged exposure. There are rare data in a pharmacodynamics
of phase II block. The purpose of this study was to
investigate a concentration-response relationship, the occurrence
and recovery of phase II block, and to clarify whether pretreatment
of succinylcholine potentiates muscle relaxation caused by rocuronium.
Methods: Hemidiaphragm-phrenic nerve preparations were dissected
from male Sprague-Dawley rats (150−250 g). Preparations were
bathed in Krebs’ solution, then maintained at 32oC, and saturated
with a mixture of 95% O2 and 5% CO2. Isometric forces made
with supramaximal stimulations (0.1 Hz, and 50 Hz for 1.9 s) to
the phrenic nerve, were measured with a force transducer, before
and after each treatment. Succinylcholine, 10, 20, 40, 60 and 80μM
were cumulatively added to the bath. Succinylcholine 80μM (for
100 min) or succinylcholine 300μM (for 20 min) was washed out.
After succinylcholine 300μM (for 20 min), or 0μM as pretreatment
was washed out, rocuronium, 2, 4, 8, 12, 16, 20μM were
cumulatively added to the bath.
Results: The potencies of single twitch, peak tetanic tension and
tetanic fade for succinylcholine were 36.1, 26.0, 20.7μM. Irrespective
of dose or exposure duration, the recovery of muscle relaxation
caused by succinylcholine was almost complete around 20 min after
succinylcholine was washed out.
Conclusions: Tetanic fade occurred at a lower concentration of
succinylcholine than single twitch. The recovery from phase II block
was relatively rapid if the concentration of succinylcholine is
sufficiently low.

영어초록

Background: Succinylcholine causes phase II block by a large
dose or a prolonged exposure. There are rare data in a pharmacodynamics
of phase II block. The purpose of this study was to
investigate a concentration-response relationship, the occurrence
and recovery of phase II block, and to clarify whether pretreatment
of succinylcholine potentiates muscle relaxation caused by rocuronium.
Methods: Hemidiaphragm-phrenic nerve preparations were dissected
from male Sprague-Dawley rats (150−250 g). Preparations were
bathed in Krebs’ solution, then maintained at 32oC, and saturated
with a mixture of 95% O2 and 5% CO2. Isometric forces made
with supramaximal stimulations (0.1 Hz, and 50 Hz for 1.9 s) to
the phrenic nerve, were measured with a force transducer, before
and after each treatment. Succinylcholine, 10, 20, 40, 60 and 80μM
were cumulatively added to the bath. Succinylcholine 80μM (for
100 min) or succinylcholine 300μM (for 20 min) was washed out.
After succinylcholine 300μM (for 20 min), or 0μM as pretreatment
was washed out, rocuronium, 2, 4, 8, 12, 16, 20μM were
cumulatively added to the bath.
Results: The potencies of single twitch, peak tetanic tension and
tetanic fade for succinylcholine were 36.1, 26.0, 20.7μM. Irrespective
of dose or exposure duration, the recovery of muscle relaxation
caused by succinylcholine was almost complete around 20 min after
succinylcholine was washed out.
Conclusions: Tetanic fade occurred at a lower concentration of
succinylcholine than single twitch. The recovery from phase II block
was relatively rapid if the concentration of succinylcholine is
sufficiently low.

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