SLA처리된 임플란트 표면의 조골세포부착에 미치는 아민 플라즈마 효과 (Effect of Amine Plasma Polymerization on Osteoblast Attachment of SLA Treated Dental Implant Surface)

서지정보

· 발행기관 : 대한치과재료학회
· 수록지 정보 : 대한치과재료학회지 / 39권 / 2호 / 111 ~ 118페이지
· 저자명 : 임성수, 이상준, 고영무

초록

Sand blast large grit acid etching (SLA) treated titanium (Ti) implant surfaces are known to enhance osteoblast activity. In this study, SLA treated Ti surface was modified by a low temperature RF discharge plasma with amine containing monomer such as allylamine (AAm), propylamine (PAAm) and evaluated in vitro cellular response. The change in wettability and surface morphology of pure Ti surface after the SLA treatment and plasma treatment were determined using contact angle measurement and field emission scanning electron microscopy. The chemical bonding state and chemical structure of amine plasma polymerized SLA/Ti surfaces were examined by Fourier transform infrared techniques and X-ray photoelectron spectroscopy. The surfaces of pure Ti, SLA treated Ti, and amine plasma polymerized SLA/Ti surfaces were also examined for their in vitro pre-osteoblast (MC3T3-E1) cells proliferation and differentiation using MTT assay and ALP activity test. The results of surface characterization showed that poly allylamine thin films was deposited on SLA/Ti surface after AAm and PAAm plasma treating for 15 min under a power of 30, 50, and 70W. The changes of surface chemistry and surface topography of Ti by AAm and PAAm plasma surface modification played main roles in improving the MC3T3-E1 cell proliferation and differentiation. These results suggest that amine containing polymeric thin films on the SLA treated Ti surface has an effect on proliferation and differentiation of MC3T3-E1 cells and potential use in osteo-conductive bone implants.

영어초록

Sand blast large grit acid etching (SLA) treated titanium (Ti) implant surfaces are known to enhance osteoblast activity. In this study, SLA treated Ti surface was modified by a low temperature RF discharge plasma with amine containing monomer such as allylamine (AAm), propylamine (PAAm) and evaluated in vitro cellular response. The change in wettability and surface morphology of pure Ti surface after the SLA treatment and plasma treatment were determined using contact angle measurement and field emission scanning electron microscopy. The chemical bonding state and chemical structure of amine plasma polymerized SLA/Ti surfaces were examined by Fourier transform infrared techniques and X-ray photoelectron spectroscopy. The surfaces of pure Ti, SLA treated Ti, and amine plasma polymerized SLA/Ti surfaces were also examined for their in vitro pre-osteoblast (MC3T3-E1) cells proliferation and differentiation using MTT assay and ALP activity test. The results of surface characterization showed that poly allylamine thin films was deposited on SLA/Ti surface after AAm and PAAm plasma treating for 15 min under a power of 30, 50, and 70W. The changes of surface chemistry and surface topography of Ti by AAm and PAAm plasma surface modification played main roles in improving the MC3T3-E1 cell proliferation and differentiation. These results suggest that amine containing polymeric thin films on the SLA treated Ti surface has an effect on proliferation and differentiation of MC3T3-E1 cells and potential use in osteo-conductive bone implants.

참고자료

· 없음