액체크로마토그라피-질량분석기(LC-MS/MS)를 이용한 소변 중 신종벤조디아제핀류 27종의 동시 분석법 개발 (Development of Simultaneous Analytical Method for 27 New Benzodiazepines in Urine Using LC-MS/MS)

서지정보

· 발행기관 : 한국법과학회
· 수록지 정보 : 한국법과학회지 / 24권 / 1호 / 1 ~ 15페이지
· 저자명 : 제갈현, 박유진, 조지영, 김신은, 강지현, 박미정

초록

Benzodiazepines (BDZ) are mainly used as a tranquilizer for anxiety, tension and depression. These drugs have apotential for abuse and addiction, serious side effects may occur when used for a long time or in combination with other centralnervous system inhibitors including alcohol. As the distribution of counterfeit medicines has increased, and new BDZ also havebeen sold on the illicit market, it is increasing possibility of abuse, and the risk of being used for crimes such as sexual assaultand gambling fraud increases. In this study, we developed a simultaneous analysis method for 27 new BDZ in urine. The urinesamples were hydrolyzed with β-glucuronidase, and extracted by solid-phase extraction (SPE). Liquid chromatography tandemmass spectrometry (LC-MS/MS) with electrospray ionization (ESI) positive mode was used for analysis. The analysis methodwas validated with respect of parameters such as limit of detection (LOD), limit of quantification (LOQ), accuracy, precision,matrix effect, recovery and efficiency. The LOD ranged from 0.5 to 5 ng/mL and LOQ ranged from 2 to 10 ng/mL. The accuracyand precision ranged from -18.5% to 13.3% and 0.2% to 18.4%, respectively. The matrix effect, recovery and efficiency rangewas 67% to 130%, 52% to 110% and 50% to 124%, respectively. Of the target substances covered in this study, 25 BDZsatisfied the validation criteria. By applying this analysis method to the forensic investigation, it could be a significant contributionto demonstrate new BDZ intoxication and abuse rapidly and accurately.

영어초록

Benzodiazepines (BDZ) are mainly used as a tranquilizer for anxiety, tension and depression. These drugs have apotential for abuse and addiction, serious side effects may occur when used for a long time or in combination with other centralnervous system inhibitors including alcohol. As the distribution of counterfeit medicines has increased, and new BDZ also havebeen sold on the illicit market, it is increasing possibility of abuse, and the risk of being used for crimes such as sexual assaultand gambling fraud increases. In this study, we developed a simultaneous analysis method for 27 new BDZ in urine. The urinesamples were hydrolyzed with β-glucuronidase, and extracted by solid-phase extraction (SPE). Liquid chromatography tandemmass spectrometry (LC-MS/MS) with electrospray ionization (ESI) positive mode was used for analysis. The analysis methodwas validated with respect of parameters such as limit of detection (LOD), limit of quantification (LOQ), accuracy, precision,matrix effect, recovery and efficiency. The LOD ranged from 0.5 to 5 ng/mL and LOQ ranged from 2 to 10 ng/mL. The accuracyand precision ranged from -18.5% to 13.3% and 0.2% to 18.4%, respectively. The matrix effect, recovery and efficiency rangewas 67% to 130%, 52% to 110% and 50% to 124%, respectively. Of the target substances covered in this study, 25 BDZsatisfied the validation criteria. By applying this analysis method to the forensic investigation, it could be a significant contributionto demonstrate new BDZ intoxication and abuse rapidly and accurately.

참고자료

· 없음