즉석섭취편의식품류에서 Staphylococcus aureus 정량검출을 위한 배지검출법과 TEMPO^® 방법과의 비교검증 (Evaluation of the Automated TEMPO^Ⓡ technique for the enumeration of Staphylococcus aureus in various ready-to-eat food products)

서지정보

· 발행기관 : 한국예방수의학회
· 수록지 정보 : 예방수의학회지 / 35권 / 4호 / 295 ~ 298페이지
· 저자명 : 송광영, 김혜성, 천정환, 김윤경, ․김동현, 성창현, 현지연, 현지연, 박준호, ․이종익, 서건호

초록

The aim of this study was to evaluate the TEMPO^Ⓡ STA automated most probable number (MPN) system for the enumeration of Staphylococcus aureus (S. aureus) in comparison with a standard culture method. Artificially inoculated food products with S. aureus - triangle kimbap, sliced spring onion, dried filefish fillet, danpatjuk (sweet red-bean porridge with small rice dumplings)- were tested in this study. Twenty-five grams of each of food samples were added into 225 ㎖ of sterilized phosphate buffered saline in a TEMPO^Ⓡ stomacher bag followed by stomaching for 2 min. One milliliter of the stomached sample was added to a bottle of culture medium. Cards were filled and sealed in the automated filler and then were incubated for 24 h at 37℃. After incubation, the cards were placed in the automated TEMPO^Ⓡ reader and MPN results were generated. For comparison with a culture method, decimal dilutions were prepared from the same homogenized samples described above, transferred onto Baird Parker and Baird Parker-Rabbit Plasma Fibrinogen (BP-RPF) agar plates, and then incubated at 37℃ for 24 h. The performance of TEMPO^Ⓡ STA method is equivalent to the culture method using Baird Parker or BP-RPF agar count plate for the enumeration of S.
aureus in foods, eliminating a time-consuming and laborious process.

영어초록

The aim of this study was to evaluate the TEMPO^Ⓡ STA automated most probable number (MPN) system for the enumeration of Staphylococcus aureus (S. aureus) in comparison with a standard culture method. Artificially inoculated food products with S. aureus - triangle kimbap, sliced spring onion, dried filefish fillet, danpatjuk (sweet red-bean porridge with small rice dumplings)- were tested in this study. Twenty-five grams of each of food samples were added into 225 ㎖ of sterilized phosphate buffered saline in a TEMPO^Ⓡ stomacher bag followed by stomaching for 2 min. One milliliter of the stomached sample was added to a bottle of culture medium. Cards were filled and sealed in the automated filler and then were incubated for 24 h at 37℃. After incubation, the cards were placed in the automated TEMPO^Ⓡ reader and MPN results were generated. For comparison with a culture method, decimal dilutions were prepared from the same homogenized samples described above, transferred onto Baird Parker and Baird Parker-Rabbit Plasma Fibrinogen (BP-RPF) agar plates, and then incubated at 37℃ for 24 h. The performance of TEMPO^Ⓡ STA method is equivalent to the culture method using Baird Parker or BP-RPF agar count plate for the enumeration of S.
aureus in foods, eliminating a time-consuming and laborious process.

참고자료

· 없음