유전자를 이용한 體質類型鑑別의 方法論에 관한 考察 (A Study on the Methodologies for the Classification of Sasang Constitution by Analysis of Genetic Polymorphism)

서지정보

· 발행기관 : 사상체질면역의학회
· 수록지 정보 : 사상체질면역의학회지 / 11권 / 2호 / 185 ~ 194페이지
· 저자명 : 하만수, 고병희, 송일병

초록

Recently some report has been published on the classification of Sasang constitution by analysis of genetic polymorphism. In this study, to present the ideas in the study on the classification of Sasang constitution based on the genetic variation of individuals, we reviewed the previous studies dealing with relationship between genetic polymorphism and Sasang constitution and suggested new molecular biological methodologies applicable to the classification of Sasang constitution. The results were as follow; 1. In the studies on the classification of constitution by analysis of genetic polymorphism, the special genes were desirable as subjects rather than the non-functional part of genome such as inter-gene and intron. Since microsatellite is usually located in inter-gene or intron, representation of constitutions may not associated with the polymorphism of certain microsatellite.
2. It may possible that polymorphism patterns of the genes related to the expression of individuality is associated with the types of Sasang constitutions. Therefore, angiotensin converting enzyme (ACE) which known have some role in the determination the human performance is one of the candidate genes can use for the classification of Sasang constitutions.
3. It is well known that HLA types are very variable in human and closely associated with the determination of susceptibility for certain disease. The studies on the polymorphism of HLA types may applicable to the classification of Sasang constitutions.
4. DNA chip technique which developed recently for the analysis of the expression of human genes may powerful tools in the study of Sasang medicine.


근래에 유전자를 이용하여 體質을 객관화하려는 시도가 있었다. 이에 본인은 그 동안 韓醫學係에서 이루어진 유전자를 이용한 四象體質의 객관화 연구와 의학계 쪽의 연구 중에서 어느 정도 연관성이 있는 논문들을 비교 검토하여 앞으로 四象醫學에서 유전자를 이용한 연구를 하는데 있어서 방향설정을 하는데 도움이 되고자 본 논문을 쓰게 되었다.
그 결과를 요약하면 다음과 같다.
1. 유전자 polymorphism(다형성)이 기능성을 가진 것을 대상으로 하여야한다.
2. Microsatellite는 기능을 가지지 않는 부분이므로 이것의 polymorphism이 體質과 관련될 가능성은 적을 것으로 사료된다.
3. Angiotensin converting enzyme (ACE)은 일반적으로 體質과 관련이 있다고 믿어지는 忍耐力을 결정하는데 중요한 유전자로 알려져 있는 바 이 효소의 polymorphism을 이용하여 體質을 분류하고자 한 시도는 의미가 있는 것으로 생각된다.
4. HLA는 다형성을 가지고 있고 HLA유전자의 발현에 따라 질병에 대한 감수성이 차이가 있다는 사실을 고려해 볼 때 앞으로 이 분야는 연구해볼 충분한 가치가 있는 것으로 생각된다.
5. DNA chip의 사용이 보편화되면 四象體質의 객관화에 많은 도움이 될 것으로 생각된다.

영어초록

Recently some report has been published on the classification of Sasang constitution by analysis of genetic polymorphism. In this study, to present the ideas in the study on the classification of Sasang constitution based on the genetic variation of individuals, we reviewed the previous studies dealing with relationship between genetic polymorphism and Sasang constitution and suggested new molecular biological methodologies applicable to the classification of Sasang constitution. The results were as follow; 1. In the studies on the classification of constitution by analysis of genetic polymorphism, the special genes were desirable as subjects rather than the non-functional part of genome such as inter-gene and intron. Since microsatellite is usually located in inter-gene or intron, representation of constitutions may not associated with the polymorphism of certain microsatellite.
2. It may possible that polymorphism patterns of the genes related to the expression of individuality is associated with the types of Sasang constitutions. Therefore, angiotensin converting enzyme (ACE) which known have some role in the determination the human performance is one of the candidate genes can use for the classification of Sasang constitutions.
3. It is well known that HLA types are very variable in human and closely associated with the determination of susceptibility for certain disease. The studies on the polymorphism of HLA types may applicable to the classification of Sasang constitutions.
4. DNA chip technique which developed recently for the analysis of the expression of human genes may powerful tools in the study of Sasang medicine.

참고자료

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