Papain 유도 호흡기 염증모델에서 인삼패독산(人蔘敗毒散)의 항염증 효과 (Anti-inflammatory effects of Insampaedok-san in papain-induced respiratory inflammation model)

서지정보

· 발행기관 : 상지대학교 한의학연구소
· 수록지 정보 : 상지한의논문집 / 11권 / 1호 / 55 ~ 65페이지
· 저자명 : 우연주, 권보인, 유준상, 이동혁, 서진우, 김주희

초록

Objectives: Insampaedok-san and its main components have been used for treatment of fever, joint pain, cough, headache and pain. Using a respiratory inflammation model, we intend to investigate the anti-inflammatory effect and its mechanism of Insampaedok-san.
Methods: We induced the actue respiratory inflammatory mouse model by papain treatment. BALB/C mice (female, 8 weeks old) were divided into three groups as follows: saline control (SC): papain treated group (vehicle): papain and Insampaedok-san (200mg/kg) treated group (n=4). To demonstrate the ani-inflammatory effect of Insampaedok-san water extracts, inflammatory cell infiltration was evaluated in bronchoalveolar lavage fluid (BALF) and nasal lavage fluid (NALF). In addition, immunogloblin (Ig)-E level was measured by using ELISA.
Results: Insampaedok-san extracts administration decreased inflammatory cell infiltration in BALF and NALF. Especially, eosinophil infiltration was dramatically reduced. Furthermore, total Ig-E level was diminished in BALF and serum by Insampaedok-san administration.
Conclusions: Our findings revealed Insampaedok-san extracts have anti-inflammatory effects by inhibiting inflammatory cell, especially eosinophil infiltration and Ig-E level.

영어초록

Objectives: Insampaedok-san and its main components have been used for treatment of fever, joint pain, cough, headache and pain. Using a respiratory inflammation model, we intend to investigate the anti-inflammatory effect and its mechanism of Insampaedok-san.
Methods: We induced the actue respiratory inflammatory mouse model by papain treatment. BALB/C mice (female, 8 weeks old) were divided into three groups as follows: saline control (SC): papain treated group (vehicle): papain and Insampaedok-san (200mg/kg) treated group (n=4). To demonstrate the ani-inflammatory effect of Insampaedok-san water extracts, inflammatory cell infiltration was evaluated in bronchoalveolar lavage fluid (BALF) and nasal lavage fluid (NALF). In addition, immunogloblin (Ig)-E level was measured by using ELISA.
Results: Insampaedok-san extracts administration decreased inflammatory cell infiltration in BALF and NALF. Especially, eosinophil infiltration was dramatically reduced. Furthermore, total Ig-E level was diminished in BALF and serum by Insampaedok-san administration.
Conclusions: Our findings revealed Insampaedok-san extracts have anti-inflammatory effects by inhibiting inflammatory cell, especially eosinophil infiltration and Ig-E level.

참고자료

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