ICR 마우스에서 황칠 추출물의 유전독성 및 단회 투여 급성 독성 (Genotoxicity and Single Oral Dose Toxicity of Dendropanax morbifera extract in ICR Mice)

서지정보

· 발행기관 : 대한미용학회
· 수록지 정보 : 대한미용학회지 / 16권 / 3호 / 231 ~ 238페이지
· 저자명 : 김진솔, 정민주, 송선영, 이현화, 정준한

초록

The current study evaluated the safety of Dendropanax morbifera extract in the ICR mouse by testing both genotoxicity and single-dose toxicity of the compound. Compared to a negative control, the micronucleus test indicated no significant increase in the number of micronucleus polychromatic erythrocytes (MNPCE) in polychromatic erythrocytes (PCE), detected in the Dendropanax morbifera extract at 250, 500, or 750 mg/kg.
Similarly, there were no changes in the rate of PCE/(PCE+NCE) in Dendropanax morbifera extract compared to the negative control group, though there was a significant decrease in the positive control group (p<0.05). In the single oral dose toxicity test, there were no significant changes in clinical signs, body weight, organ weights or gross observation compared to the negative control group. The serum biochemical examination indicated that compared to the negative control group, the ALP level was significantly increased in female mice at doses of 500 and 750 mg/kg of Dendropanax morbifera extract (p<0.05), however there was no significant difference in the correlated liver index test items such as AST and ALP. Histopathological observations revealed no change between treatment and negative control groups in liver or kidney tissues. Taken together, these results found no observed adverse effect level (NOAEL) of the single oral application of Dendropanax morbifera extract up to 750 mg/kg in mice. Based on these findings, we suggest that Dendropanax morbifera could be used as a non-toxic natural material for functional cosmetics, food, and medicine development.

영어초록

The current study evaluated the safety of Dendropanax morbifera extract in the ICR mouse by testing both genotoxicity and single-dose toxicity of the compound. Compared to a negative control, the micronucleus test indicated no significant increase in the number of micronucleus polychromatic erythrocytes (MNPCE) in polychromatic erythrocytes (PCE), detected in the Dendropanax morbifera extract at 250, 500, or 750 mg/kg.
Similarly, there were no changes in the rate of PCE/(PCE+NCE) in Dendropanax morbifera extract compared to the negative control group, though there was a significant decrease in the positive control group (p<0.05). In the single oral dose toxicity test, there were no significant changes in clinical signs, body weight, organ weights or gross observation compared to the negative control group. The serum biochemical examination indicated that compared to the negative control group, the ALP level was significantly increased in female mice at doses of 500 and 750 mg/kg of Dendropanax morbifera extract (p<0.05), however there was no significant difference in the correlated liver index test items such as AST and ALP. Histopathological observations revealed no change between treatment and negative control groups in liver or kidney tissues. Taken together, these results found no observed adverse effect level (NOAEL) of the single oral application of Dendropanax morbifera extract up to 750 mg/kg in mice. Based on these findings, we suggest that Dendropanax morbifera could be used as a non-toxic natural material for functional cosmetics, food, and medicine development.

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