바퀴(Blattella germanica)에서 발현되는 세린계 단백분해효소 유전자군의 염기서열분석 및 알레르겐으로서의 가능성 (Cloning of a Family of Serine Protease Genes Expressed in German Cockroach (Blattella germanica) and Possibility as Allergens)

서지정보

· 발행기관 : 대한이비인후과학회
· 수록지 정보 : 대한이비인후-두경부외과학회지 / 51권 / 6호 / 528 ~ 534페이지
· 저자명 : 이원용, 김현범, 박성현, 박경원, 옥미선, 유태현, 박종률

초록

The serine protease allergens have been known as one of the most important inhalent allergens
of insects as well as house dust mites. There is no known serine protease allergen of German cockroach, which is a well-known
etiological agent of allergic diseases. We identified the serine protease genes of cockroach, Blattella germanica, and examined its
possibility as an allergen. Materials and Method：We have isolated three partial genes, Bg3, Bg5 and Bg6, of serine protease
from the Blattella germanica cDNA library using the degenerate oligonucleotide PCR primers specific for the conserved regions.
Results：The genes, Bg3, Bg5 and Bg6, were composed of 396 bp 131 amino acids, 513 bp 170 amino acids, and 410 bp 136
amino acids, respectively. Northern hybridization analysis indicated that the size of Bg5 and Bg6 transcripts was approximately
0.7 kb. The gene, Bg5, was also identified as a multiple gene by the Southern blot analysis. The gene, Bg3, showed significant
homology to trypsin-like serine protease of various insects. The deduced amino acids sequence of the gene Bg5 was matched to
the mite group III allergen as well as various species of insect serine protease sequences. The gene Bg6 also showed high homology
to the amino acids sequences of insect serine proteases and mite group III allergens. Three Blattella germanica serine protease
gene fragments revealed close genetic relationships with Dermatophagoides farinae group III allergen in the phylogenetic analysis.
Conclusion：The high homology and close phylogenetic relationship raise the possibility of three serine protease genes as being
an allergen of German cockroach. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:528-34)

영어초록

The serine protease allergens have been known as one of the most important inhalent allergens
of insects as well as house dust mites. There is no known serine protease allergen of German cockroach, which is a well-known
etiological agent of allergic diseases. We identified the serine protease genes of cockroach, Blattella germanica, and examined its
possibility as an allergen. Materials and Method：We have isolated three partial genes, Bg3, Bg5 and Bg6, of serine protease
from the Blattella germanica cDNA library using the degenerate oligonucleotide PCR primers specific for the conserved regions.
Results：The genes, Bg3, Bg5 and Bg6, were composed of 396 bp 131 amino acids, 513 bp 170 amino acids, and 410 bp 136
amino acids, respectively. Northern hybridization analysis indicated that the size of Bg5 and Bg6 transcripts was approximately
0.7 kb. The gene, Bg5, was also identified as a multiple gene by the Southern blot analysis. The gene, Bg3, showed significant
homology to trypsin-like serine protease of various insects. The deduced amino acids sequence of the gene Bg5 was matched to
the mite group III allergen as well as various species of insect serine protease sequences. The gene Bg6 also showed high homology
to the amino acids sequences of insect serine proteases and mite group III allergens. Three Blattella germanica serine protease
gene fragments revealed close genetic relationships with Dermatophagoides farinae group III allergen in the phylogenetic analysis.
Conclusion：The high homology and close phylogenetic relationship raise the possibility of three serine protease genes as being
an allergen of German cockroach. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:528-34)

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