동소이식 유방암 근적외선 이미징을 이용한 우스닉산 칼륨염의 항암 효능 평가 (Evaluation of Anti-cancer Efficacy of Potassium Usnate using NIR Imaging of Orthotopic Breast Cancer Mouse Model)

서지정보

· 발행기관 : 대한약학회
· 수록지 정보 : 약 학 회 지 / 66권 / 5호 / 278 ~ 282페이지
· 저자명 : 손예선, 이창욱, 유인탁, 이미진, 김항건

초록

Mouse cancer models are useful tools for evaluating in vivo tumor growth and metastasis, providing valuableinformation for preclinical testing. In this process, optical imaging enables the mouse models to easily identify the progressof disease in a non-invasive way. Here, we established an experimental bioimaging animal model of near-infrared (NIR)fluorescence by using a fluorescence-labeled organism bioimaging instrument (FOBI) and evaluated the anti-cancer effectof potassium usnate (KU) in an orthotopic breast cancer model. The cell viability assay revealed that KU had cytotoxicitywith half maximal inhibitory concentration of approximately 138.57, 167.69, and 144.17 μM in 4T1-Fluc-Neo/iRFP-Puro(4T1-iRFP), MDA-MB-231, and MCF-7 cells, respectively. The measurement of NIR fluorescence from the 4T1-iRFPcells in a microtube via FOBI exhibited a strong correlation between cell number and fluorescence intensity, and theminimal detection limit was 105 cells. Accordingly, NIR imaging was performed on the orthotopic breast cancer mousemodel by using FOBI, and regression of tumor progression through intraperitoneal KU administration was successfullymonitored. Our results demonstrated the establishment of NIR imaging in the orthotopic breast cancer animal model forevaluating the anti-cancer effect of KU.

영어초록

Mouse cancer models are useful tools for evaluating in vivo tumor growth and metastasis, providing valuableinformation for preclinical testing. In this process, optical imaging enables the mouse models to easily identify the progressof disease in a non-invasive way. Here, we established an experimental bioimaging animal model of near-infrared (NIR)fluorescence by using a fluorescence-labeled organism bioimaging instrument (FOBI) and evaluated the anti-cancer effectof potassium usnate (KU) in an orthotopic breast cancer model. The cell viability assay revealed that KU had cytotoxicitywith half maximal inhibitory concentration of approximately 138.57, 167.69, and 144.17 μM in 4T1-Fluc-Neo/iRFP-Puro(4T1-iRFP), MDA-MB-231, and MCF-7 cells, respectively. The measurement of NIR fluorescence from the 4T1-iRFPcells in a microtube via FOBI exhibited a strong correlation between cell number and fluorescence intensity, and theminimal detection limit was 105 cells. Accordingly, NIR imaging was performed on the orthotopic breast cancer mousemodel by using FOBI, and regression of tumor progression through intraperitoneal KU administration was successfullymonitored. Our results demonstrated the establishment of NIR imaging in the orthotopic breast cancer animal model forevaluating the anti-cancer effect of KU.

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