석회화 전처리가 양극산화 처리한 타이타늄 임플란트의 생체활성도에 미치는 영향 (Effect of Precalcification Treatment on Bioactivity of Anodized Titanium Implant)

서지정보

· 발행기관 : 대한치과재료학회
· 수록지 정보 : 대한치과재료학회지 / 38권 / 4호 / 263 ~ 272페이지
· 저자명 : 박소영, 박일송, 오상훈, 정회웅, 소윤조, 김형섭

초록

This study was performed to improve the bioactivity of anodized nanotubular TiO2 layer. The glycerol solution containing 1 wt% NH4F and 20 wt% deionized water was used as an electrolyte. Pulse signals with a potential of 20 V and current density of 20 mA/cm2 were applied for 60 minutes. Nanotubular TiO2 layer was heat-treated at 500℃ for 2 hours and precalcified by soaking in Na2HPO4 solution at room temperature for 24 hours and then in saturated Ca(OH)2 solution for 5 hours. The samples were divided into 4 groups:(1) pure Ti; (2) anodized Ti; (3) anodized and heat-treated; and (4) anodized, heat-treated and precalcified.
The 36 implants were placed in both tibiae of 18 rats. After implantation periods of 2 and 4 weeks, the degree of osseointegration of implants were evaluated by micro CT analysis, western blotting and RT-PCR.
Micro CT images revealed more dense appearance around screw part of implants in the anodized,heat-treated, and precalcified group than in other groups. Bone mineral density and trabecular density values exhibited coherent results with same oder. Collagen Type I and 2 as well as osteocalcin which are involved in bone remodeling also exhibited the highest expression in the anodized, heat-treated, &precalcified group than in other groups. In conclusion, anodized, heat-treated, and precalcified titanium implants significantly improved osseointegration ability in compared to others.

영어초록

This study was performed to improve the bioactivity of anodized nanotubular TiO2 layer. The glycerol solution containing 1 wt% NH4F and 20 wt% deionized water was used as an electrolyte. Pulse signals with a potential of 20 V and current density of 20 mA/cm2 were applied for 60 minutes. Nanotubular TiO2 layer was heat-treated at 500℃ for 2 hours and precalcified by soaking in Na2HPO4 solution at room temperature for 24 hours and then in saturated Ca(OH)2 solution for 5 hours. The samples were divided into 4 groups:(1) pure Ti; (2) anodized Ti; (3) anodized and heat-treated; and (4) anodized, heat-treated and precalcified.
The 36 implants were placed in both tibiae of 18 rats. After implantation periods of 2 and 4 weeks, the degree of osseointegration of implants were evaluated by micro CT analysis, western blotting and RT-PCR.
Micro CT images revealed more dense appearance around screw part of implants in the anodized,heat-treated, and precalcified group than in other groups. Bone mineral density and trabecular density values exhibited coherent results with same oder. Collagen Type I and 2 as well as osteocalcin which are involved in bone remodeling also exhibited the highest expression in the anodized, heat-treated, &precalcified group than in other groups. In conclusion, anodized, heat-treated, and precalcified titanium implants significantly improved osseointegration ability in compared to others.

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