국소뇌허혈 후 허혈경계영역에서의 Activating Transcription Factor 3의 발현 (Expression of Activating Transcription Factor 3 in Ischemic Penumbra Region Following Focal Cerebral Ischemia)

서지정보

· 발행기관 : 대한해부학회
· 수록지 정보 : Anatomy and Cell Biology / 41권 / 3호 / 173 ~ 183페이지
· 저자명 : 송대용, 오경민, 이지혜, 우란숙, 이윤정, 한정태, 백태경

초록

It has been demonstrated that some of immediate early genes (IEGs) such as c-Jun or fos are induced
immediately following neuronal injury and they play an important role in determining the fate of the injured neurons.
Of IEGs, the activating transcription factor 3 (ATF3) is focused by many investigators, because they are expressed in
various types of neural insults and have been known to serve a diverse function in both neuronal survival and death.
However, little is known about the functional role of ATF3 in ischemic brain injury. So in this study, the authors
examined the expression pattern of the activating transcription factor 3 (ATF3) following middle cerebral artery (MCA)
occlusion-reperfusion injury. According to the findings obtained by triphenyltetrazolium chloride (TTC) stains, the
authors have classified the infarcted area into two regions, the ischemic core region and the ischemic penumbra region.
In both regions, many neurons underwent neuronal degeneration, characterized by the shrunken nuclei with eosinophilic
perikaryon. The H & E stain also demonstrated the increased number of probable activated astrocytes and microglia
in the ischemic brain regions and this was confirmed by GFAP- and OX42-immunohistochemistry. Immunohistochemical
study for ATF3 also demonstrated the specific upregulation of ATF3 in the nuclei of neurons under ischemic
injury, but not in those of the contralateral regions. Interestingly, the number of the ATF3 positive neurons in the ischemic
penumbra regions outnumbered that of the ischemic core regions. Based on many reports that the neuronal death
in ischemic penumbra region is caused by programed cell death rather than by necrosis which is main cause of neuronal
death in ischemic core region, our results could suggest that the ATF3 is an important IEGs which determine the
fate of the ischemic neurons.

영어초록

It has been demonstrated that some of immediate early genes (IEGs) such as c-Jun or fos are induced
immediately following neuronal injury and they play an important role in determining the fate of the injured neurons.
Of IEGs, the activating transcription factor 3 (ATF3) is focused by many investigators, because they are expressed in
various types of neural insults and have been known to serve a diverse function in both neuronal survival and death.
However, little is known about the functional role of ATF3 in ischemic brain injury. So in this study, the authors
examined the expression pattern of the activating transcription factor 3 (ATF3) following middle cerebral artery (MCA)
occlusion-reperfusion injury. According to the findings obtained by triphenyltetrazolium chloride (TTC) stains, the
authors have classified the infarcted area into two regions, the ischemic core region and the ischemic penumbra region.
In both regions, many neurons underwent neuronal degeneration, characterized by the shrunken nuclei with eosinophilic
perikaryon. The H & E stain also demonstrated the increased number of probable activated astrocytes and microglia
in the ischemic brain regions and this was confirmed by GFAP- and OX42-immunohistochemistry. Immunohistochemical
study for ATF3 also demonstrated the specific upregulation of ATF3 in the nuclei of neurons under ischemic
injury, but not in those of the contralateral regions. Interestingly, the number of the ATF3 positive neurons in the ischemic
penumbra regions outnumbered that of the ischemic core regions. Based on many reports that the neuronal death
in ischemic penumbra region is caused by programed cell death rather than by necrosis which is main cause of neuronal
death in ischemic core region, our results could suggest that the ATF3 is an important IEGs which determine the
fate of the ischemic neurons.

참고자료

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