고량강 추출물의 암세포증식 저해 효과 (Anti-proliferative Effect of the Rhizome Extract of Alpinia officinarum on Cultured Human Tumor Cell Lines)

서지정보

· 발행기관 : 한국생약학회
· 수록지 정보 : 생약학회지 / 39권 / 4호 / 347 ~ 351페이지
· 저자명 : 이호성, 차미란, 최춘환, 최상운, 김영섭, 김영균, 김영호, 연규환, 유시용

초록

The methanol (MeOH) extract of the rhizome of Alpinia officinarum Hance (Zingiberaceae) demonstrated a potent
inhibition on the proliferation of cultured human tumor cell lines such as MES-SA (human uterine carcinoma cell line), MESSA/
DX5 (multidrug resistant subline of MES-SA), HCT-15 (human colorectal adenocarcinoma cell line), HCT15/CL02 (multidrug
resistant subline of HCT15). The MeOH extract was fractionated into four portions by serial solvent partition, ie., methylene
chloride (CH2Cl2) soluble part, ethylacetate (EtOAc) soluble part, n-butanol (BuOH) soluble part and remaining water
layer. Among them, the CH2Cl2 soluble part of the extract exhibited a most potent inhibition on the proliferation of tested tumor
cell lines. Bioassay-guided fractionation of the CH2Cl2 soluble part led to the isolation of five diarylheptanoid and two flavonoid
constituents, i. e., galangin (1), 7-(4"-hydroxy-3"-methoxyphenyl)-1-phenylhept-4-en-3-one (2), 1,7-diphenyl-5-hydroxy-3-heptanone
(3), trans,trans-1-(3'-methoxy-4'-hydroxyphenyl)-7-phenyl-5-ol-4,6-dien-3-heptanone (4), 5-methoxy-7-(4"-hydroxy-3"-
methoxyphenyl)-1-phenyl-3-heptanone (5), kaempferide (6), 5-hydroxy-7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-3-heptanone
(7). Structures of the isolated active components (1 - 7) were established by chemical and spectroscopic means.

영어초록

The methanol (MeOH) extract of the rhizome of Alpinia officinarum Hance (Zingiberaceae) demonstrated a potent
inhibition on the proliferation of cultured human tumor cell lines such as MES-SA (human uterine carcinoma cell line), MESSA/
DX5 (multidrug resistant subline of MES-SA), HCT-15 (human colorectal adenocarcinoma cell line), HCT15/CL02 (multidrug
resistant subline of HCT15). The MeOH extract was fractionated into four portions by serial solvent partition, ie., methylene
chloride (CH2Cl2) soluble part, ethylacetate (EtOAc) soluble part, n-butanol (BuOH) soluble part and remaining water
layer. Among them, the CH2Cl2 soluble part of the extract exhibited a most potent inhibition on the proliferation of tested tumor
cell lines. Bioassay-guided fractionation of the CH2Cl2 soluble part led to the isolation of five diarylheptanoid and two flavonoid
constituents, i. e., galangin (1), 7-(4"-hydroxy-3"-methoxyphenyl)-1-phenylhept-4-en-3-one (2), 1,7-diphenyl-5-hydroxy-3-heptanone
(3), trans,trans-1-(3'-methoxy-4'-hydroxyphenyl)-7-phenyl-5-ol-4,6-dien-3-heptanone (4), 5-methoxy-7-(4"-hydroxy-3"-
methoxyphenyl)-1-phenyl-3-heptanone (5), kaempferide (6), 5-hydroxy-7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-3-heptanone
(7). Structures of the isolated active components (1 - 7) were established by chemical and spectroscopic means.

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