Effect of Monosodium Glutamate on In Vitro Oocyte Maturation and Embryonic Development after Parthenogenesis in Pigs

서지정보

· 발행기관 : 한국수정란이식학회
· 수록지 정보 : Journal of Embryo Transfer / 32권 / 4호
· 저자명 : Minji Kim, Hyeji Shin, Joohyeong Lee, Eunsong Lee

목차

INTRODUCTION
MATERIALS and METHODS
1. Culture Media and Reagents
2. Oocyte Collection and IVM
3. Experimental Design
4. Parthenogenetic Activation (PA) and In Vitro Culture of PA embryos
5. Determination of Intra-Oocyte GSH content and Cumulus Cell Expansion after IVM
6. Statistical Analysis
RESULTS
1. Effect of MSG in a Non-Essential Amino Acids (NEAA)-Free Chemically Defined Medium on Oocyte Maturation and PA Embryonic Development (Experiment 1)
2. Effect of MSG in a Non-Essential Amino Acids (NEAA)-Free Chemically Defined Medium on Intra-Oocyte GSH contents and Cumulus Expansion (Experiment 2)
3. Effect of MSG in a Chemically Defined Medium Containing Non-Essential Amino Acids (NEAA) on oocyte maturation and PA embryonic development (Experiment 3)
4. Effect of MSG during IVC in a Non-Essential Amino Acids (NEAA)-Free Chemically Defined Medium on PA Embryonic Development (Experiment 4)
DISCUSSION
ACKNOWLEDGMENTS
REFERENCES

영어초록

This study was designed to determine the effect of monosodium glutamate (MSG) on in vitro maturation (IVM) of oocytes and early development of parthenogenesis (PA) embryos in pigs. Each IVM and IVC medium was supplemented with various concentrations (0, 0.1, 0.5 and 5 mM) of MSG and non-essential amino acids (NEAA) depending on the experimental design. Immature pig oocytes were matured for 44 h and then oocytes reached metaphase II (MII) stage were electrically activated to induce parthenogenesis (PA). When immature oocytes were treated with MSG in the absence of NEAA during IVM, nuclear maturation (83.1-87.1%), intra-oocyte glutathione content, cumulus expansion, and cleavage (91.4-93.4%) of PA embryos were not influenced by MSG treatment at all concentrations. However, blastocyst formation of PA embryos was significantly increased by 5.0 mM MSG (45.3 ± 6.2%) compared to control (25.6 ± 3.4%). MSG treatment during IVM in the presence of NEAA did not show significant effect on nuclear maturation of oocytes and blastocyst formation after PA while 0.5 mM MSG (89.3 ± 1.9%) decreased (P < 0.05) cleavage of PA embryos compared to 0.1 mM MSG (94.6 ± 1.1%). When PA embryos were treated for 7 days with MSG during IVC, 5.0 mM MSG significantly decreased blastocyst formation (27.8 ± 4.9%) compared to no treatment (41.4 ± 1.9%) while no decrease in blastocyst formation was observed in 0.1 and 0.5 mM (37.4 ± 3.4% and 34.4 ± 2.6%, respectively). Our results demonstrated that 5 mM MSG in a NEAA-free chemically defined maturation medium showed positive effect on PA embryonic development while 5 mM MSG treatment during IVC was deleterious to PA embryonic development in pigs.

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