Peptoniphilus mikwangii -specific quantitative real-time polymerase chain reaction primers
(주)코리아스칼라
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- 2023.04.03
- 최종 저작일
- 2019.09
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서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 44권 / 3호
ㆍ저자명 : Soon-Nang Park, Joong-Ki Kook
목차
Introduction
Materials and Methods
1. 세균 및 세균 배양
2. quantitative real-time polymerase chain reaction(qPCR) 프라이머 설계 및 종-특이성 검증
3. quantitative real-time polymerase chain reaction(qPCR)을 이용한 민감도 조사
Results
Discussion
References
영어 초록
The purpose of this study was to develop Peptoniphilus mikwangii -specific quantitative real-time polymerase chain reaction (qPCR) primers based on the 16S ribosomal RNA (16S rDNA) gene. The specificity of the primers was determined by conventional PCR using 29 strains of 27 oral bacterial species including P. mikwangii. The sensitivity of the primers was determined by qPCR using the purified genomic DNA of P. mikwangii KCOM 1628T (40 ng to 4 fg). The data showed that the qPCR primers (RTB134-F4/RTB134-R4) could detect P. mikwangii strains exclusively and as little as 40 fg of the genomic DNA of P. mikwangii KCOM 1628T. These results suggest that the developed qPCR primer pair can be useful for detecting P. mikwangii in epidemiological studies of oral bacterial infectious diseases.
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