Development of strain-specific polymerase chain reaction primers to detect Fusobacterium hwasookii strains

서지정보

· 발행기관 : 대한구강생물학회
· 수록지 정보 : International Journal of Oral Biology / 46권 / 4호
· 저자명 : Yun Kyong Lim, Joong-Ki Kook

목차

Introduction
Materials and Methods
1. 세균 및 세균 배양
2. F . hwasookii 5개 균주에 대한 균주-특이 PCR프라이머 쌍 설계
3. F . hwasookii 5개 균주에 대한 균주-특이 PCR프라이머들의 종-특이성 및 민감도(검출 한계) 검증
Results
Discussion
References

영어초록

This study aimed to develop strain-specific polymerase chain reaction (PCR) primers to detect Fusobacterium hwasookii KCOM 1249T, F. hwasookii KCOM 1253, F. hwasookii KCOM 1256, F. hwasookii KCOM 1258, and F. hwasookii KCOM 1268 on the basis of nucleotide sequences of a gene specific to each strain. The unique genes for each F. hwasookii strain were determined on the basis of their genome sequences using Roary. The strain-specific PCR primers based on each strain-specific gene were designed using PrimerSelect. The specificity of each PCR primer was determined using the genomic DNA of the 5 F. hwasookii strains and 25 strains of oral bacterial species. The detection limit and sensitivity of each strain-specific PCR primer pair were determined using the genomic DNA of each target strain. The results showed that the strain-specific PCR primers correspond to F. hwasookii KCOM 1249T, F. hwasookii KCOM 1253, F. hwasookii KCOM 1258, F. hwasookii KCOM 1256/F. nucleatum subsp. polymorphum KCOM 1260, or F. hwasookii KCOM 1268/Fusobacterium sp. oral taxon 203 were developed. The detection limits of these strain-specific PCR primers ranged from 0.2 to 2 ng of genomic DNA for each target strain. The results suggest that these strain-specific PCR primers are valuable in quality control for detecting specific F. hwasookii strains.

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