Bis(2-ethylhexyl) phthalate가 in vitro에서 식물 토양병원성 세균 Pectobacterium carotovorum 에 미치는 영향

서지정보

· 발행기관 : 한국환경생물학회
· 수록지 정보 : 환경생물 / 40권 / 4호
· 저자명 : 김유리, 김상태, 상미경

목차

Abstract
서 론
재료 및 방법
1. 세균 배양과 개체군 평가
2. TCA cycle
3. 막 투과성과 ATPase 활성
4. 병원성 관련 유전자 발현
5. 통계분석
결과 및 고찰
적 요
사 사
REFERENCES

영어초록

Bis(2-ethylhexyl) phthalate (DEHP) is one of the plasticizers used in the polyvinyl chloride (PVC) industry. It is known to be easily released into the environment. In this study, we investigated effects of DEHP on growth, metabolic pathway, and virulence gene expression in soil-borne bacterial plant pathogen, Pectobacterium carotovorum SCC1 using in vitro assays. As a result, DEHP at 20 μg mL-1 did not affect the growth, cell membrane permeability, or ATPase activity of P. carotovorum SCC1. However, it decreased succinyl-CoA synthase (SCS) activity in the tricarboxylic acid (TCA) cycle. Relative expression levels of virulence genes encoding pectate lyase and pectin were differentially influenced by DEHP treatment. These results suggest that biological characteristics of P. carotovorum might be influenced by DEHP in soil.

참고자료

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