개 미성숙난자의 체내이식 배양이 핵성숙에 미치는 영향

서지정보

· 발행기관 : 한국수정란이식학회
· 수록지 정보 : Journal of Embryo Transfer / 20권 / 1호
· 저자명 : 이효상, 이영호, 윤희준, 공일근

초록

본 연구는 개의 미성숙난자의 낮은 성숙율을 개선하기 위하여 자연발정온 개의 생식기관에 미성숙 난자를 이식하여 체내 배양 난자를 회수하여 회수된 난자의 핵 성숙율을 조사한 결과는 다음과 같다. 1. 미성숙난자를 24, 48 및 72시간 동안 체외성숙을 유도하였을 때 성숙시간에 따른 체외성숙율에는 유의적 차이는 인정되지 않았다. 2. 체내 배양 기간에 따른 난자의 회수율은 배양기간이 길어질수록 유의적으로 낮은 회수율을 보였으며, 배양기간이 증가할수록 난자의

영어초록

This study were carried out to evaluate the possibility of nuclear progression of canine immature oocytes, of which was cultured in a reproductive tract, such as oviduct, ovarian bursa and uterus of estrus bitch for 4, 5 and 6 days following immediately collection. Cumulus intact oocytes(COC) fore collected from domestic dog following ovariohysterectomy at local veterinary clinics. In Exp. 1, COCs of>110 μm diameter were selected and cultured in vitro at , in air atmosphere. The nuclear progression of canine oocytes checked at 24, 48 or 72 h after in vitro maturation. There was not increased the nuclear progression to the M II stage depending on culture periods at 24, 48 and 72h . In Exp. 2, to evaluate of nuclear progression of immature oocytes, collected or in vitro cultured oocytes were transfer into a canine reproductive tract (oviduct, ovarian bursa and uterus). The recovery rates of canine oocytes from a reproductive tract after 4 days in vivo culture were significantly higher than those 5 6 day (P<0.05). The survival rates of collected oocytes after 4 days were also significantly higher than those of 5 days and 6 days (P<0.05). The meiotic resumption rates of canine oocytes were not significantly difference among the culture periods at 4 days , 5 days and 6 days . These results show that the nuclear progression of canine immature oocytes from in in vivo culture was not affect the nuclear resumption of oocytes.

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