돼지정액의 동결에 관한 연구 II. 동결한 돼지정액의 체내, 체외수정능력

서지정보

· 발행기관 : 한국수정란이식학회
· 수록지 정보 : Journal of Embryo Transfer / 20권 / 1호
· 저자명 : 김광식, 송해범

초록

본 실험은 LEY에 다양한 당류를 첨가한 용액으로 동결보존한 돼지정자의 응해후 생존율과 정상 첨체율, 체외성숙한 난자와의 수정능력과 배 발달 능력 및 인공수정을 통한 수태율과 산자수에 미치는 효과를 조사하기 위해 실시하였다. 융해후의 정상 첨체율은 glucose 농도 증가와 함께 증가되었으나, 생존율에 있어서는 효과가 인정되지 않았다. LEY에 fructose를 단독 또는 glucose와 함께 첨가하면 융해후의 생존율이 유의하게 증가되었으며 (p<0.0

영어초록

This experiment was carried out to investigate the effects of saccharide in the lactose-egg yolk(LEY) extender for freezing of boar semen on the viability, normal acrosome, fertilizable of in vitro or in vivo oocyte after thawed. Normal acrosome post-thawed spermatozoa was higher when increasing of glucose concentration in LEY extender with 3 or glycerol, but viability was not significant. Viability of the post-thawed spermatozoa was higher when fructose or fructose and glucose were added to LEY extender with glycerol than glucose and sucrose or fructose, glucose and sucrose(P<0.05). Rate of normal acrosome of post thawed spermatozoa was higher when both fructose and glucose were added to the LEY extender than saccharide not added to it(P<0.001). The percentage of fertilization, cleavage and development to blastocyst of oocytes fertilized with post-thawed spermatozoa from freezing by LEY extender were , and , respectively. Conception rate by artificial insemination with frozen boa. semen was higher than commercial frozen semen from SGI company(50.0±0.1%, P<0.05), but litter size were no significant differences between frozen by LEY extender and SGI semen.

참고자료

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