Effects of BSA, PVA, Gonadotropins and Follicle Shell on In Vitro Maturation and In Vitro Fertilization of Porcine Oocytes

서지정보

· 발행기관 : 한국동물번식학회
· 수록지 정보 : Reproductive & developmental biology / 31권 / 2호
· 저자명 : Pei-Qing Cong, Eun-Sook Song, Eui-Sook Kim, Zhao-Hua Li, Yong-Hua Zhang, Young-Joo Yi, Chang-Sik Park

목차

ABSTRACT INTRIDUSTION MATERIALS AND METHODS Oocyte Collection and In Vitro maturation(IVM) Semen Collection and Liquid Semen Processing In Vitro Fertilization(IVF) and Culture of Oocytes Evaluation of Oocytes and Embryos Produced In Vitro Experimental Designs Statistical Analysis RESULTS Effects of Different mTCM-199 Media on IVM Effects of BSA and PVA and IVM Effects of BSA and PVA on IVF and Subsequent Development Effects of Gonadotropins on IVM Effects of Follicle Shell Co-culture on IVM, and Embryos Development DISCUSSION REFERENCES

영어초록

This study was designed to evaluate effects of BSA, PVA, gonadotropins and follicle shell during IVM of porcine oocytes and subsequent development to the blastocyst stage after IVF. Cumulus oocyte complexes (COCs) were cultured in TCM-199 media containing 4 mg/ml BSA and 1 mg/ml PVA during IVM for 44 hr. To compare the effect of gonadotropins on oocyte maturation, COCs were cultured with FSH+LH, FSH, LH and FSH-LH-free media during IVM, respectively. Also, different number of follicle shells (0, 2, 4 and 6) was used to examine whether the presence of follicle shell in culture medium affects oocyte maturation. The percentages of fertilization and blastocyst formation, respectively, were higher in the medium containing the PVA (49.0 and 17.9%) than those containing the BSA (40.0 and 12.2%). Significantly higher rates of MII oocytes were in the presence of FSH+LH and FSH (88.6 and 85.1%) compared to other treatments (64.0 and 53.4% at LH and FSH-LH-free media). Co-culture with inverted follicle shells in 2 ml maturation medium enhanced the developmental competence of porcine oocytes. In conclusion, PVA could be used as a macromolecules instead of BSA, and FSH and follicle shell played important roles in maturation of porcine oocytes.

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