복강경을 이용한 한국흑염소의 성주기 판정

서지정보

· 발행기관 : 한국수정란이식학회
· 수록지 정보 : Journal of Embryo Transfer / 22권 / 1호
· 저자명 : 양홍석, 장성근, 용환율, 조종기, 신상태

초록

본 연구는 복강경을 이용하여 한국흑염소의 난소를 연속적으로 관찰하여 그 형태적 변화를 확인함으로써 한국흑염소의 성주기를 판정하고 그 결과를 한국흑염소의 수정란 이식시에 활용하고자 하는 목적으로 수행하였다. 제거 2일 후부터 복강경 수술을 통해 난소의 형태적 변화를 관찰하였다. 난소의 크기와 황체의 변화를 관찰하였으며, 황체는 그 성숙과 퇴축 과정에 따라 corpus hemorrhagicum(CH), corpus luteum(CL), corpus albi

영어초록

This study was performed to determine the estrous cycles by macroscopic observation of the ovarian changes using the laparoscopy and to make use of these results for embryo transfer in Korean black goat (Copra hircus aegagrus). Laparoscopic examinations of the ovaries were performed from 2 days after removal to 22 days after ovulation. The serial morphological changes of follicles and corpus luteum (CL) were observed. CL was classified corpus hemorrhagicum(CH), corpus luteum (CL) and corpus albicans (CA) by its maturation and regression. On the day before ovulation (Day 0), Graafian follicles (GF) were found on one or both ovaries. On the day (Day 1) and day (Day 2) of ovulation, and ovulation depression (OD) and an early stage corpus hemorrhagicum were observed at the site of GF, respectively. On Days 3 to 4, more developed and enlarged corpus hemorrhagicum arised from the ovulation of the GF with well vascularization. On Days 5 to 6, it was identified that mature corpus luteum was grown on the ovary, and fully developed CL with adjacent follicles were occupied most part of the ovary on Days 17 and 18. Then the size of CL was diminished, and completely luteal regression with new large follicle was identified on Days 20 and 22. From these results, the 4 stages of the estrous cycle in Korean black goats were 1) estrus (Day 0) for 1 day, 2) metestrus for 4 days (stage of CH development), 3) diestrus for 12 or 13 days (luteal stage), and 4) proestrus for 4 or 5 days (stage of luteal regression and follicular growing). Laparoscopy for observation of ovarian changes was invasive than laparotomy. Additionally, it had advantages of reduced adhesion and quick operation time. It was considered that laparoscopic examination of ovarian changes will be useful for embryo transfer in the Korean black goats.

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